Methylation of viral and host genes and severity of cervical lesions associated with human papillomavirus type 16

Methylation of human papillomavirus (HPV) and host genes may predict cervical cancer risk. We examined the methylation status of selected sites in HPV16 and human genes in DNA extracted from exfoliated cervical cell samples of 244 women harboring HPV16‐positive cancer or cervical intraepithelial neo...

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Bibliographic Details
Published in:International journal of cancer 2015-03, Vol.136 (6), p.E638-E645
Main Authors: Louvanto, Karolina, Franco, Eduardo L., Ramanakumar, Agnihotram V., Vasiljević, Nataša, Scibior‐Bentkowska, Dorota, Koushik, Anita, Cuzick, Jack, Coutlée, Francois, Lorincz, Attila T.
Format: Article
Language:English
Subjects:
Adult
biomarker
Cancer
Cervical cancer
cervical intraepithelial neoplasia (CIN)
Deoxyribonucleic acid
DNA
DNA Methylation
Female
HPV16
Human papillomavirus
human papillomavirus (HPV)
Human papillomavirus 16
Human papillomavirus 16 - genetics
Humans
Medical research
ROC Curve
Severity of Illness Index
Uterine Cervical Dysplasia - genetics
Uterine Cervical Dysplasia - virology
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - virology
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Summary:Methylation of human papillomavirus (HPV) and host genes may predict cervical cancer risk. We examined the methylation status of selected sites in HPV16 and human genes in DNA extracted from exfoliated cervical cell samples of 244 women harboring HPV16‐positive cancer or cervical intraepithelial neoplasia (CIN) or negative for intraepithelial lesions or malignancy (NILM). We quantified the methylation of CpG sites in the HPV16 L1 gene (CpG 6367 and 6389) and in the human genes EPB41L3 (CpG 438, 427, 425) and LMX1 (CpG 260, 262, 266, 274) following bisulfite treatment and pyrosequencing. Receiver operating characteristic (ROC) curves were used to analyze the diagnostic utility of methylation level for the different sites and for a joint predictor score. Methylation in all sites significantly increased with lesion severity (p 
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.29196