Investigating the mode of action of sulfoxaflor: a fourth‐generation neonicotinoid

BACKGROUND: The precise mode of action of sulfoxaflor, a new nicotinic acetylcholine receptor‐modulating insecticide, is unclear. A detailed understanding of the mode of action, especially in relation to the neonicotinoids, is essential for recommending effective pest management practices. RESULTS:...

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Published in:Pest management science 2013-05, Vol.69 (5), p.607-619
Main Authors: Cutler, Penny, Slater, Russell, Edmunds, Andrew JF, Maienfisch, Peter, Hall, Roger G, Earley, Fergus GP, Pitterna, Thomas, Pal, Sitaram, Paul, Verity‐Laura, Goodchild, Jim, Blacker, Melissa, Hagmann, Leonhard, Crossthwaite, Andrew J
Format: Article
Language:English
Subjects:
Animals
Aphididae
Aphids
Binding sites
Binding, Competitive
Biological and medical sciences
Control
Fundamental and applied biological sciences. Psychology
Imidacloprid
insecticidal properties
Insecticide Resistance
Insecticides
Insecticides - toxicity
Insects
mechanism of action
Mutation
Myzus persicae
nervous system
nicotinic acetylcholine receptor
nitenpyram
Pest control
pest management
Phytopathology. Animal pests. Plant and forest protection
point mutation
Protozoa. Invertebrates
Pyridines - chemical synthesis
Pyridines - toxicity
radiolabel
Receptors, Nicotinic - chemistry
resistance
resistance management
Sulfur Compounds - chemical synthesis
Sulfur Compounds - toxicity
Tritium
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Summary:BACKGROUND: The precise mode of action of sulfoxaflor, a new nicotinic acetylcholine receptor‐modulating insecticide, is unclear. A detailed understanding of the mode of action, especially in relation to the neonicotinoids, is essential for recommending effective pest management practices. RESULTS: Radiolabel binding experiments using a tritiated analogue of sulfoxaflor ([³H]‐methyl‐SFX) performed on membranes from Myzus persicae demonstrate that sulfoxaflor interacts specifically with the high‐affinity imidacloprid binding site present in a subpopulation of the total nAChR pool. In competition studies, imidacloprid‐like neonicotinoids displace [³H]‐methyl‐SFX at pM concentrations. The effects of sulfoxaflor on the exposed aphid nervous system in situ are analogous to those of imidacloprid and nitenpyram, and finally the high‐affinity sulfoxaflor binding site is absent in a Myzus persicae strain (clone FRC) possessing a single amino acid point mutation (R81T) in the β‐nAChR, a region critical for neonicotinoid interaction. CONCLUSION: The nicotinic acetylcholine receptor pharmacological profile of sulfoxaflor in aphids is consistent with that of imidacloprid. Additionally, the insecticidal activity of sulfoxaflor and the current commercialised neonicotinoids is affected by the point mutation in FRC Myzus persicae. Therefore, it is suggested that sulfoxalfor be considered a neonicotinoid, and that this be taken into account when recommending insecticide rotation partnering for effective resistance management programmes. © 2012 Society of Chemical Industry
ISSN:1526-498X
1526-4998
DOI:10.1002/ps.3413