Quercetin Prevents DNA Single Strand Breakage and Cytotoxicity Caused By tert-Butylhydroperoxide: Free Radical Scavenging Versus Iron Chelating Mechanism

Although the antioxidant properties of flavonoids are well documented, it is still unclear whether these effects are dependent on radical scavenging or iron chelating activities. By using an experimental approach based on the notion that iron chelators suppress DNA strand scission and cytotoxicity c...

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Bibliographic Details
Published in:Free radical biology & medicine 1998-07, Vol.25 (2), p.196-200
Main Authors: Sestili, Piero, Guidarelli, Andrea, Dachà, Marina, Cantoni, Orazio
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
Butylated Hydroxytoluene - pharmacology
Cell Death - drug effects
Chromans - pharmacology
Copper
Cytotoxicity
DNA damage
DNA Damage - drug effects
Dose-Response Relationship, Drug
Flavonoids
Free radical
Free Radical Scavengers - pharmacology
Humans
Hydrogen peroxide
Iron Chelating Agents - pharmacology
Leukemia, Myeloid
Phenanthrolines - pharmacology
Phenylenediamines - pharmacology
Quercetin
Quercetin - pharmacology
tert-Butylhydroperoxide
tert-Butylhydroperoxide - antagonists & inhibitors
tert-Butylhydroperoxide - toxicity
Tumor Cells, Cultured
Vitamin E - pharmacology
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Summary:Although the antioxidant properties of flavonoids are well documented, it is still unclear whether these effects are dependent on radical scavenging or iron chelating activities. By using an experimental approach based on the notion that iron chelators suppress DNA strand scission and cytotoxicity caused by tert-butylhydroperoxide, whereas radical scavenging antioxidants prevent only the latter response, we provide experimental evidence indicating that the most prominent activity of the flavonoid quercetin resides in its ability to chelate iron. This experimental approach can be utilized for the assessment of iron chelation in the biological activity of flavonoids or other antioxidants.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(98)00040-9