Characterization of resistance genes in 68 ESBL-producing Klebsiella pneumonia in Lebanon

We studied 68 ESBL-producing Klebsiella pneumoniae strains isolated in Lebanon, determined their profile of resistance to antibiotics, and identified 6 ESBL genes. The susceptibility to antibiotics was determined by the disk diffusion method. The MIC of carbapenems and cefepime was determined by the...

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Bibliographic Details
Published in:Médecine et maladies infectieuses 2014-12, Vol.44 (11-12), p.535-538
Main Authors: Obeid Charrouf, F., Hamze, M., Mallat, H., Achkar, M., Dabboussi, F.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
beta-Lactamases - genetics
beta-Lactamases - metabolism
Biological and medical sciences
BLSE
Carbapenems - pharmacology
Cephalosporins - pharmacology
Colony Count, Microbial
Disk Diffusion Antimicrobial Tests
Drug Resistance, Multiple, Bacterial - genetics
ESBL
Genes, Bacterial
Humans
Infectious diseases
Klebsiella Infections - epidemiology
Klebsiella Infections - microbiology
Klebsiella pneumonia
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Lebanon
Lebanon - epidemiology
Liban
Medical sciences
Microbial Sensitivity Tests
Organ Specificity
Pneumology
Respiratory system : syndromes and miscellaneous diseases
Substrate Specificity
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Summary:We studied 68 ESBL-producing Klebsiella pneumoniae strains isolated in Lebanon, determined their profile of resistance to antibiotics, and identified 6 ESBL genes. The susceptibility to antibiotics was determined by the disk diffusion method. The MIC of carbapenems and cefepime was determined by the agar dilution method. ESBL genes were detected by PCR. A percentage of 88.2% and 86.7% of isolates carried the SHV and CTX-M gene respectively; combinations of more than 1 gene of resistance were detected in several isolates. Five strains were resistant to carbapenems; 4/5 carried the OXA-48 gene. Our study revealed the emergence of K. pneumoniae ESBL (+) strains carrying several types of genes involved in this phenotype; we also identified carbapenem-resistant strains due to the OXA-48 gene, which are a real threat for public health, especially in Lebanon. Étude de 68 isolats cliniques de Klebsiella pneumoniae productrices de bêta-lactamases à spectre élargi (BLSE), isolées au Liban. Détermination du profil de résistance aux antibiotiques et détection de 6 gènes de BLSE. La sensibilité aux antibiotiques a été déterminée par la méthode de diffusion. La CMI a été déterminée par la méthode de dilution en milieu solide. Les gènes de BLSE ont été détectés par PCR. Un pourcentage de 88,2 % et 86,7 % des isolats portaient le gène SHV et CTX-M respectivement ; des combinaisons de gènes de résistance ont été détectées. Cinq souches étaient résistantes aux carbapénèmes, 4/5 hébergeaient le gène OXA-48. L’étude a révélé l’émergence des souches de K. pneumoniae BLSE (+) qui portaient plusieurs types de gènes de résistance ; à cela s’ajoutait le problème des souches résistantes aux carbapénèmes par le gène OXA-48 qui constitue une vrai menace pour la santé publique.
ISSN:0399-077X
1769-6690
DOI:10.1016/j.medmal.2014.10.001