An Investigation of Freezing of Supercooled Water on Anti-Freeze Protein Modified Surfaces

This work investigates how functionalization of aluminium surfaces with natural type III Anti-Freeze Protein （AFP） affects the mechanism of heterogeneous ice nucleation. First the bulk ice nucleation properties of distilled water and aqueous solution of AFP were evaluated by differential scanning ca...

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Bibliographic Details
Published in:Journal of bionics engineering 2013-04, Vol.10 (2), p.139-147
Main Authors: Charpentier, Thibaut VJ, Neville, Anne, Millner, Paul, Hewson, Rob, Morina, Ardian
Format: Article
Language:English
Subjects:
Aluminium
Aluminum
anti-freeze protein
Artificial Intelligence
Biochemical Engineering
Bioinformatics
Biomaterials
Biomedical Engineering and Bioengineering
Biomedical Engineering/Biotechnology
Droplets
Engineering
Fourier transforms
Freezing
ice nucleation temperature
ice-like monolayer
Nucleation
Proteins
Secondary ion mass spectroscopy
surface functionalization
二次离子质谱仪
傅立叶变换红外光谱
冷冻温度
冻结
差示扫描量热法
抗冻蛋白
表面改性
过冷水
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Summary:This work investigates how functionalization of aluminium surfaces with natural type III Anti-Freeze Protein （AFP） affects the mechanism of heterogeneous ice nucleation. First the bulk ice nucleation properties of distilled water and aqueous solution of AFP were evaluated by differential scanning calorimetry. Then the modified surface was characterized by Secondary Ions Mass Spectroscopy （SIMS）, Fourier Transform InfraRed （FTIR） spectroscopy and contact angle measurement. Freezing experiments were then conducted in which water droplets underwent a slow controlled cooling. This study shows that compared to uncoated aluminium, the anti-freeze proteins functionalized surfaces exhibit a higher and narrower range of freezing temperature. It was found that these proteins that keep living organisms from freezing in cold environment act in the opposite way once immobilized on surfaces by promoting ice nucleation. Some suggestions regarding the mechanism of action of the observed phenomena were proposed based on the Classical Nucleation Theory （CNT）.
ISSN:1672-6529
2543-2141
DOI:10.1016/S1672-6529(13)60208-5