The effect of gold nanoparticle size on osteogenic differentiation of adipose-derived stem cells

[Display omitted] •GNPs promoted the differentiation of ADSCs toward osteoblasts.•The uptake increased in the order of 100, 75, 15, 30, and 50nm sizes.•30 and 50nm GNPs were preferentially up taken into ADSCs.•30 and 50nm GNPs were effective at enhancing osteogenic differentiation of ADSCs. There ha...

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Bibliographic Details
Published in:Journal of colloid and interface science 2015-01, Vol.438, p.68-76
Main Authors: Ko, Wan-Kyu, Heo, Dong Nyoung, Moon, Ho-Jin, Lee, Sang Jin, Bae, Min Soo, Lee, Jung Bok, Sun, In-Cheol, Jeon, Hoon Bong, Park, Hun Kuk, Kwon, Il Keun
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - drug effects
Assaying
Biocompatibility
Biomedical materials
Cell Differentiation - drug effects
Differentiation
Gold
Gold - chemistry
Gold - pharmacology
Gold nanoparticles
Human adipose-derived stem cell
Humans
Metal Nanoparticles
Nanoparticles
Osteoblasts
Osteogenesis
Osteogenesis - physiology
Osteogenic differentiation
Particle Size
Real-Time Polymerase Chain Reaction
Stem cells
Stem Cells - cytology
Stem Cells - drug effects
Uptake
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Summary:[Display omitted] •GNPs promoted the differentiation of ADSCs toward osteoblasts.•The uptake increased in the order of 100, 75, 15, 30, and 50nm sizes.•30 and 50nm GNPs were preferentially up taken into ADSCs.•30 and 50nm GNPs were effective at enhancing osteogenic differentiation of ADSCs. There have been many medical applications based on gold nanoparticles (GNPs) over the past several centuries. Recently, researchers have focused on bone tissue engineering applications utilizing GNPs. The effect of various sizes of gold nanoparticles on the differentiation of human adipose-derived stem cells (ADSCs) into osteoblasts was investigated. The concentration of gold nanoparticles was fixed at 1μM and varying sizes of 15, 30, 50, 75 and 100nm (spherical GNPs) were used. The lack of cytotoxicity was confirmed by establishing viability of ADSCs using cell counting kit-8 (CCK-8) and live/dead assays. The results showed that each size of GNPs had no significant toxicity on ADSCs during 1week of incubation. Osteogenic differentiation of ADSCs was confirmed by alkaline phosphatase (ALP) staining, ALP activity, calcium deposition, and real time PCR experiments. It was found, through dark field assays and microscope cell images, that 30nm and 50nm GNPs were preferentially up taken into the ADSCs. As expected, all sizes of gold nanoparticles promoted the differentiation of ADSCs toward osteoblasts more than control. Among all sizes, 30 and 50nm GNPs appeared to have the highest differentiation rates. The data consistently demonstrated that 30 and 50nm GNPs are the most effective in promoting osteogenic differentiation of ADSCs.
ISSN:0021-9797
1095-7103
DOI:10.1016/j.jcis.2014.08.058