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Non-enzymatic electrochemical detection of cholesterol using β-cyclodextrin functionalized graphene
A non-enzymatic approach towards cholesterol detection is presented here, exploiting the electrochemical non-enzymatic route of sensing which has a distinct advantage over other conventional enzymatic processes. Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and h...
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| Published in: | Biosensors & bioelectronics 2015-01, Vol.63, p.212-217 |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c452t-70aecc1dbc2643da33a3d039e836f35c436ca54e802bef13c10853eec01308f3 |
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| cites | cdi_FETCH-LOGICAL-c452t-70aecc1dbc2643da33a3d039e836f35c436ca54e802bef13c10853eec01308f3 |
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| container_title | Biosensors & bioelectronics |
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| creator | Agnihotri, Nidhi Chowdhury, Ankan Dutta De, Amitabha |
| description | A non-enzymatic approach towards cholesterol detection is presented here, exploiting the electrochemical non-enzymatic route of sensing which has a distinct advantage over other conventional enzymatic processes. Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and high surface area material, provides a platform for the electrochemical detection of cholesterol using Methylene Blue as redox indicator. Methylene Blue (MB) forms an inclusion complex with Grp-β-CD and emerges as a cholesterol sensing matrix. MB molecule is replaced by cholesterol molecule and moves out in the buffer solution, hence, detected electrochemically using Differential Pulse Voltammetric (DPV) technique. The sensing matrix is characterised using FT-IR and Raman spectroscopy. Transmission Electron Microscopy is carried out to study the morphology of functionalized graphene sheets.
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•Non-enzymatic route of cholesterol sensing, using MB as redox indicator.•Better solubility of Grp-β-CD-MB (sensing probe) due to hydrophilic interactions.•Entrapment of cholesterol inside Grp-β-CD-MB cavity, forming an inclusion complex.•Cholesterol detection using DPV in a wide range of 1–100µM and high sensitivity. |
| doi_str_mv | 10.1016/j.bios.2014.07.037 |
| format | article |
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[Display omitted]
•Non-enzymatic route of cholesterol sensing, using MB as redox indicator.•Better solubility of Grp-β-CD-MB (sensing probe) due to hydrophilic interactions.•Entrapment of cholesterol inside Grp-β-CD-MB cavity, forming an inclusion complex.•Cholesterol detection using DPV in a wide range of 1–100µM and high sensitivity.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.07.037</identifier><identifier>PMID: 25104431</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>beta-Cyclodextrins - chemistry ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; Buffer solutions ; Cholesterol ; Cholesterol - analysis ; Cholesterol - chemistry ; Cholesterol sensing ; Conductometry - instrumentation ; Cyclic Voltammetery ; Detection ; Differential Pulse Voltammetery ; Enzymes - chemistry ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Graphene ; Graphene-β-Cyclodextrin ; Graphite - chemistry ; Inclusion complex ; Methylene blue ; Platforms ; Redox indicator ; Surface area</subject><ispartof>Biosensors & bioelectronics, 2015-01, Vol.63, p.212-217</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-70aecc1dbc2643da33a3d039e836f35c436ca54e802bef13c10853eec01308f3</citedby><cites>FETCH-LOGICAL-c452t-70aecc1dbc2643da33a3d039e836f35c436ca54e802bef13c10853eec01308f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28831745$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25104431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Agnihotri, Nidhi</creatorcontrib><creatorcontrib>Chowdhury, Ankan Dutta</creatorcontrib><creatorcontrib>De, Amitabha</creatorcontrib><title>Non-enzymatic electrochemical detection of cholesterol using β-cyclodextrin functionalized graphene</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A non-enzymatic approach towards cholesterol detection is presented here, exploiting the electrochemical non-enzymatic route of sensing which has a distinct advantage over other conventional enzymatic processes. Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and high surface area material, provides a platform for the electrochemical detection of cholesterol using Methylene Blue as redox indicator. Methylene Blue (MB) forms an inclusion complex with Grp-β-CD and emerges as a cholesterol sensing matrix. MB molecule is replaced by cholesterol molecule and moves out in the buffer solution, hence, detected electrochemically using Differential Pulse Voltammetric (DPV) technique. The sensing matrix is characterised using FT-IR and Raman spectroscopy. Transmission Electron Microscopy is carried out to study the morphology of functionalized graphene sheets.
[Display omitted]
•Non-enzymatic route of cholesterol sensing, using MB as redox indicator.•Better solubility of Grp-β-CD-MB (sensing probe) due to hydrophilic interactions.•Entrapment of cholesterol inside Grp-β-CD-MB cavity, forming an inclusion complex.•Cholesterol detection using DPV in a wide range of 1–100µM and high sensitivity.</description><subject>beta-Cyclodextrins - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Buffer solutions</subject><subject>Cholesterol</subject><subject>Cholesterol - analysis</subject><subject>Cholesterol - chemistry</subject><subject>Cholesterol sensing</subject><subject>Conductometry - instrumentation</subject><subject>Cyclic Voltammetery</subject><subject>Detection</subject><subject>Differential Pulse Voltammetery</subject><subject>Enzymes - chemistry</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Graphene</subject><subject>Graphene-β-Cyclodextrin</subject><subject>Graphite - chemistry</subject><subject>Inclusion complex</subject><subject>Methylene blue</subject><subject>Platforms</subject><subject>Redox indicator</subject><subject>Surface area</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkc-O0zAQhy0EYruFF-CAckHikjDO2E4icUEr_kkruOzdcp3J1lViFztBdB9rH4RnwqUFbsBppNE3o59-H2PPOFQcuHq1qzYupKoGLipoKsDmAVvxtsFS1CgfshV0UpVSKbxglyntAKDhHTxmF7XkIATyFes_BV-SvztMZna2oJHsHIPd0uSsGYue5rxwwRdhKOw2jJRmimEsluT8bfH9vrQHO4aevs3R-WJY_E_ajO6O-uI2mv2WPD1hjwYzJnp6nmt28-7tzdWH8vrz-49Xb65LK2Q9lw0Yspb3G1srgb1BNNgDdtSiGlBagcoaKaiFekMDR8uhlUhkgSO0A67Zy9PbfQxflpxUTy5ZGkfjKSxJcyW54E2j2v9AUQJCp_DfqJSdqnmd-1yz-oTaGFKKNOh9dJOJB81BH5XpnT4q00dlGhqdleWj5-f_y2ai_vfJL0cZeHEGTMpOhmi8dekP17bIGyEz9_rEUa74q6Ook3XkLfUuZom6D-5vOX4A4Fe2bQ</recordid><startdate>20150115</startdate><enddate>20150115</enddate><creator>Agnihotri, Nidhi</creator><creator>Chowdhury, Ankan Dutta</creator><creator>De, Amitabha</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20150115</creationdate><title>Non-enzymatic electrochemical detection of cholesterol using β-cyclodextrin functionalized graphene</title><author>Agnihotri, Nidhi ; Chowdhury, Ankan Dutta ; De, Amitabha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-70aecc1dbc2643da33a3d039e836f35c436ca54e802bef13c10853eec01308f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>beta-Cyclodextrins - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Buffer solutions</topic><topic>Cholesterol</topic><topic>Cholesterol - analysis</topic><topic>Cholesterol - chemistry</topic><topic>Cholesterol sensing</topic><topic>Conductometry - instrumentation</topic><topic>Cyclic Voltammetery</topic><topic>Detection</topic><topic>Differential Pulse Voltammetery</topic><topic>Enzymes - chemistry</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. 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Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and high surface area material, provides a platform for the electrochemical detection of cholesterol using Methylene Blue as redox indicator. Methylene Blue (MB) forms an inclusion complex with Grp-β-CD and emerges as a cholesterol sensing matrix. MB molecule is replaced by cholesterol molecule and moves out in the buffer solution, hence, detected electrochemically using Differential Pulse Voltammetric (DPV) technique. The sensing matrix is characterised using FT-IR and Raman spectroscopy. Transmission Electron Microscopy is carried out to study the morphology of functionalized graphene sheets.
[Display omitted]
•Non-enzymatic route of cholesterol sensing, using MB as redox indicator.•Better solubility of Grp-β-CD-MB (sensing probe) due to hydrophilic interactions.•Entrapment of cholesterol inside Grp-β-CD-MB cavity, forming an inclusion complex.•Cholesterol detection using DPV in a wide range of 1–100µM and high sensitivity.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>25104431</pmid><doi>10.1016/j.bios.2014.07.037</doi><tpages>6</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | beta-Cyclodextrins - chemistry Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Buffer solutions Cholesterol Cholesterol - analysis Cholesterol - chemistry Cholesterol sensing Conductometry - instrumentation Cyclic Voltammetery Detection Differential Pulse Voltammetery Enzymes - chemistry Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Graphene Graphene-β-Cyclodextrin Graphite - chemistry Inclusion complex Methylene blue Platforms Redox indicator Surface area |
| title | Non-enzymatic electrochemical detection of cholesterol using β-cyclodextrin functionalized graphene |
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