Non-enzymatic electrochemical detection of cholesterol using β-cyclodextrin functionalized graphene

A non-enzymatic approach towards cholesterol detection is presented here, exploiting the electrochemical non-enzymatic route of sensing which has a distinct advantage over other conventional enzymatic processes. Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and h...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-01, Vol.63, p.212-217
Main Authors: Agnihotri, Nidhi, Chowdhury, Ankan Dutta, De, Amitabha
Format: Article
Language:English
Subjects:
beta-Cyclodextrins - chemistry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
Buffer solutions
Cholesterol
Cholesterol - analysis
Cholesterol - chemistry
Cholesterol sensing
Conductometry - instrumentation
Cyclic Voltammetery
Detection
Differential Pulse Voltammetery
Enzymes - chemistry
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Graphene
Graphene-β-Cyclodextrin
Graphite - chemistry
Inclusion complex
Methylene blue
Platforms
Redox indicator
Surface area
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Summary:A non-enzymatic approach towards cholesterol detection is presented here, exploiting the electrochemical non-enzymatic route of sensing which has a distinct advantage over other conventional enzymatic processes. Chemically converted Graphene modified with β-CD, being hydrophilic, electroactive and high surface area material, provides a platform for the electrochemical detection of cholesterol using Methylene Blue as redox indicator. Methylene Blue (MB) forms an inclusion complex with Grp-β-CD and emerges as a cholesterol sensing matrix. MB molecule is replaced by cholesterol molecule and moves out in the buffer solution, hence, detected electrochemically using Differential Pulse Voltammetric (DPV) technique. The sensing matrix is characterised using FT-IR and Raman spectroscopy. Transmission Electron Microscopy is carried out to study the morphology of functionalized graphene sheets. [Display omitted] •Non-enzymatic route of cholesterol sensing, using MB as redox indicator.•Better solubility of Grp-β-CD-MB (sensing probe) due to hydrophilic interactions.•Entrapment of cholesterol inside Grp-β-CD-MB cavity, forming an inclusion complex.•Cholesterol detection using DPV in a wide range of 1–100µM and high sensitivity.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.07.037