Green synthesis, characterization and anti-inflammatory activity of silver nanoparticles using European black elderberry fruits extract

•A completely green method for the synthesis of silver nanoparticles was developed.•Polyphenols from European black elderberry fruit extract were used as reducing, stabilizing and capping agents.•The synthesized nanoparticles were found to possess in vitro anti-inflammatory activity.•AgNPs also exhi...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2014-10, Vol.122, p.767-777
Main Authors: David, Luminita, Moldovan, Bianca, Vulcu, Adriana, Olenic, Liliana, Perde-Schrepler, Maria, Fischer-Fodor, Eva, Florea, Adrian, Crisan, Maria, Chiorean, Ioana, Clichici, Simona, Filip, Gabriela Adriana
Format: Article
Language:English
Subjects:
Adoxaceae
Adoxaceae family
Anti-Inflammatory Agents - pharmacology
Anti-inflammatory effect
Biomedical materials
Cell Line, Transformed
Cytokines
Elderberries
European black elderberry (Sambucus nigra)
Humans
In vitro testing
In vivo tests
Metal Nanoparticles
Microscopy, Electron, Transmission
Nanoparticles
Plant Extracts - pharmacology
Psoriasis
Sambucus - chemistry
Sambucus nigra
Silver
Silver - chemistry
Silver nanoparticles
Spectroscopy, Fourier Transform Infrared
Surgical implants
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Summary:•A completely green method for the synthesis of silver nanoparticles was developed.•Polyphenols from European black elderberry fruit extract were used as reducing, stabilizing and capping agents.•The synthesized nanoparticles were found to possess in vitro anti-inflammatory activity.•AgNPs also exhibited promising in vivo anti-inflammatory properties. This research aimed at reporting the synthesis, characterization and evaluation of the anti-inflammatory effects of some new biomaterials based on silver nanoparticles and polyphenols rich natural extracts. A fast and eco-friendly extracellular biosynthesis of silver nanoparticles (AgNPs), using European black elderberry (Sambucus nigra – SN, Adoxaceae family) fruit extracts was developed. The phytosynthesized nanoparticles exhibited an absorbance peak at 426nm, characteristic for AgNPs and their sizes were ranged from 20 to 80nm. The anti-inflammatory properties of AgNPs were assessed in vitro on HaCaT cells exposed to UVB radiation, in vivo on acute inflammation model and in humans on psoriasis lesions. In vitro, our results demonstrated the anti-inflammatory effects of functionalized AgNPs by the decrease of cytokines production induced by UVB irradiation. In vivo, the pre-administration of AgNPs reduced the edema and cytokines levels in the paw tissues, early after the induction of inflammation. The present study also demonstrated the possible use of synthesized AgNPs for the treatment of psoriasis lesions.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2014.08.018