Leptospira species categorized by arbitrarily primed polymerase chain reaction (PCR) and by mapped restriction polymorphisms in PCR-amplified rRNA genes

Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene)....

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Bibliographic Details
Published in:Journal of Bacteriology 1993-02, Vol.175 (4), p.973-981
Main Authors: Ralph, D. (California Institute of Biological Research, La Jolla, CA), McClelland, M, Welsh, J, Baranton, G, Perolat, P
Format: Article
Language:English
Subjects:
ADN
ARN RIBOSOMAL
ARN RIBOSOMIAL
Bacteriology
Base Sequence
BIOCHIMIE
Biological and medical sciences
BIOQUIMICA
CHIMIOTAXONOMIE
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
FILOGENIA
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Bacterial
Genetics
LEPTOSPIRA
Leptospira - classification
Leptospira - genetics
Microbiology
Molecular Sequence Data
Oligodeoxyribonucleotides - chemistry
PHYLOGENIE
Phylogeny
POLIMORFISMO
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
POLYMORPHISME
QUIMIOTAXONOMIA
Ribonucleic acid
RIBOSOMAS
RIBOSOME
RNA
RNA, Ribosomal - genetics
Sequence Alignment
SEROTIPOS
SEROTYPE
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Description
Summary:Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 length polymorphisms were used to group reference strains into 16 MRSP profiles. Species assignments were consistent with those obtained by a second method, genomic fingerprinting with arbitrarily primed PCR, in which strains within a species were characterized by many shared arbitrarily primed PCR products. The results of both of these methods were in general agreement with those of previous studies that used DNA-DNA relatedness and confirmed the high level of divergence among the recognized species of Leptospira. However, Leptospira meyeri serovar ranarum and evansi strains were indistinguishable from some strains of Leptospira interrogans sensu stricto. Intervening sequences of about 485 to 740 bp were located near base 1230 in rrl of some strains
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.4.973-981.1993