Effects of manganese deficiency on chondrocyte development in tibia growth plate of Arbor Acres chicks

The aim of this study was to investigate the effects of manganese (Mn) deficiency on chondrocyte development in tibia growth plate. Ninety 1-day-old Arbor Acres chicks were randomly divided into three groups and fed on control diet (60 mg Mn/kg diet) and manganese deficient diets (40 mg Mn/kg diet,...

Full description

Saved in:
Bibliographic Details
Published in:Journal of bone and mineral metabolism 2015-01, Vol.33 (1), p.23-29
Main Authors: Wang, Jian, Wang, Zhen Yong, Wang, Zhao Jun, Liu, Ran, Liu, Shao Qiong, Wang, Lin
Format: Article
Language:English
Subjects:
Animal Feed
Animals
Apoptosis
Cell Differentiation
Chickens
Chondrocytes - cytology
Cyclin-Dependent Kinase Inhibitor p21 - metabolism
Gene Expression Regulation
Growth Plate - cytology
Immunohistochemistry
In Situ Nick-End Labeling
Manganese - chemistry
Manganese - deficiency
Medicine
Medicine & Public Health
Metabolic Diseases
Original Article
Orthopedics
Proto-Oncogene Proteins c-bcl-2 - metabolism
Real-Time Polymerase Chain Reaction
RNA, Messenger - metabolism
Tibia - growth & development
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to investigate the effects of manganese (Mn) deficiency on chondrocyte development in tibia growth plate. Ninety 1-day-old Arbor Acres chicks were randomly divided into three groups and fed on control diet (60 mg Mn/kg diet) and manganese deficient diets (40 mg Mn/kg diet, manganese deficiency group I; 8.7 mg Mn/kg diet, manganese deficiency group II), respectively. The width of the proliferative zone of growth plate was measured by the microscope graticule. Chondrocyte apoptosis was estimated by TUNEL staining. Gene expression of p21 and Bcl-2, and expression of related proteins were analyzed by quantitative real time reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Compared with the control group, manganese deficiency significantly decreased the proliferative zone width and Bcl-2 mRNA expression level, while significantly increased the apoptotic rates and the expression level of p21 gene in chondrocytes. The results indicate that manganese deficiency had a negative effect on chondrocyte development, which was mediated by the inhibition of chondrocyte proliferation and promotion of chondrocyte apoptosis.
ISSN:0914-8779
1435-5604
DOI:10.1007/s00774-014-0563-0