Isolation and Characterization of Cadmium- and Arsenic-Absorbing Bacteria for Bioremediation

The contamination of hazardous metal(loid) is one of the serious environmental and human health risks. This study isolated a total of 40 cadmium (Cd)- and arsenic (As)-resistant bacterial isolates from coastal sediments by pour plate technique using tryptic soy agar supplemented with Cd or As (50 mg...

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Published in:Water, air, and soil pollution air, and soil pollution, 2014-10, Vol.225 (10), p.1-10, Article 2151
Main Authors: Bhakta, Jatindra N, Munekage, Y, Ohnishi, K, Jana, B. B, Balcazar, J. L
Format: Article
Language:English
Subjects:
Acinetobacter
Analysis
aquatic environment
Arsenic
Atmospheric Protection/Air Quality Control/Air Pollution
Bacteria
Bioremediation
Cadmium
Chemical properties
Climate Change/Climate Change Impacts
Coastal
culture media
Earth and Environmental Science
effluents
Environment
Exiguobacterium
Health aspects
human health
Hydrogeology
Magnesium
minimum inhibitory concentration
nucleotide sequences
phylogeny
Planococcus
Pseudomonas
Pseudomonas abietaniphila
ribosomal DNA
risk
sediments
Sediments (Geology)
Soil Science & Conservation
Water Quality/Water Pollution
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Summary:The contamination of hazardous metal(loid) is one of the serious environmental and human health risks. This study isolated a total of 40 cadmium (Cd)- and arsenic (As)-resistant bacterial isolates from coastal sediments by pour plate technique using tryptic soy agar supplemented with Cd or As (50 mg l⁻¹) for use as metal(loid) bioremediation agents. Out of 40, 4 isolates, RCd3, RCd6, RAs7, and RAs10, showed a relatively higher growth rate in Cd- or As-supplemented culture media which were selected for further study. The selected isolates showed a high minimum inhibitory concentration (60–400 mg l⁻¹for Cd and 400–2200 mg l⁻¹for As), which demonstrated their remarkable Cd and As resistance capabilities. The metal(loid) removal efficiencies (0.032–0.268 μg Cd h⁻¹ mg⁻¹and 0.0003–0.0172 μg As h⁻¹ mg⁻¹[wet weight cell]) of selected isolates indicated their greater magnitude in absorbing Cd compared to As from water. Phylogenetic analysis of the 16S rDNA sequences revealed that isolates RCd3, RCd6, RAs7, and RAs10 were closely related to Acinetobacter brisouii, Pseudomonas abietaniphila, Exiguobacterium aestuarii, and Planococcus rifietoensis, respectively. Because of high Cd and As resistance and removal efficiency, the selected isolates can survive in a high metal(loid)-contaminated environment and could be a potential tool for bioremediation of high metal(loid)-contaminated effluents to protect the aquatic environment.
ISSN:0049-6979
1573-2932
DOI:10.1007/s11270-014-2151-2