Supercritical fluid chromatography in research laboratories: Design, development and implementation of an efficient generic screening for exploiting this technique in the achiral environment

► Achiral SFC screen designed, developed and successfully implemented (routine). ► Approach developed in support of purification in chemistry groups in Drug Discovery. ► Success: >85% targets resolved from its impurities in hundreds of research mixtures. ► Scale-up: >90% recovery and >95% p...

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Bibliographic Details
Published in:Journal of Chromatography A 2011-11, Vol.1218 (47), p.8551-8560
Main Authors: de la Puente, M.L., López Soto-Yarritu, P., Burnett, J.
Format: Article
Language:English
Subjects:
Achiral purification
Analysis
Analytical screen
Arenas
Biological and medical sciences
Chromatography
Drug Discovery
drugs
Elution
General pharmacology
Mathematical analysis
Medical sciences
Pharmacology. Drug treatments
Purification
Screening
Strategy
Supercritical Fluid Chromatography
Supercritical fluids
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Summary:► Achiral SFC screen designed, developed and successfully implemented (routine). ► Approach developed in support of purification in chemistry groups in Drug Discovery. ► Success: >85% targets resolved from its impurities in hundreds of research mixtures. ► Scale-up: >90% recovery and >95% purity for isolated target. ► Scope, generality, efficiency and cost effectiveness fully proven. A new strategy for exploiting SFC in the achiral arena has been designed, developed and successfully implemented in our Analytical Technologies laboratories, in support of Drug Discovery. The development of a generic analytical SFC/MS screening protocol, following extensive evaluation of different stationary phase, modifier and gradient elution combinations, has enabled fast analytical method development for structurally diverse compounds within both specialised and high-throughput purification environments. Application of this approach into the daily routine has provided data on a large number and a wide variety of reaction mixtures. The results confirm the scope and generality of the strategy with more than 85% of targets being successfully resolved from their impurities.
ISSN:0021-9673
DOI:10.1016/j.chroma.2011.09.029