Peptide-based electrochemical approach for apoptosis evaluation

This paper reports a strategy to assemble apoptotic cells on a solid surface using a peptide as the recognition element. And a peptide-based electrochemical biosensor to directly evaluate apoptosis is described for the first time. The peptide modified on an electrode is designed to contain the seque...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2014-12, Vol.62, p.97-101
Main Authors: Miao, Peng, Yin, Jian, Ning, Limin, Li, Xiaoxi
Format: Article
Language:English
Subjects:
Apoptosis
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensor
Biosensors
Biotechnology
Electrochemical Techniques - instrumentation
Electrochemistry
Electrodes
Equipment Design
Fundamental and applied biological sciences. Psychology
Humans
MCF-7 Cells
Methods. Procedures. Technologies
Peptide
Peptides
Peptides - chemistry
Phosphatidylserine
Phosphatidylserines - analysis
Recognition
Shields
Strategy
Various methods and equipments
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Summary:This paper reports a strategy to assemble apoptotic cells on a solid surface using a peptide as the recognition element. And a peptide-based electrochemical biosensor to directly evaluate apoptosis is described for the first time. The peptide modified on an electrode is designed to contain the sequence that can recognize externalized phosphatidylserine on apoptotic cells, and can then capture the cells onto the electrode surface. In the electrochemical system, the immobilized cells can not only provide significant steric hindrance for electron transfer, but also shield the positive charges of the peptide that can attract negatively charged electrochemical probes. Therefore, the obtained electrochemical signals drop significantly after the incubation of apoptotic cells, which can be used to reveal the apoptosis level. The experimental results of this approach are well in line with other standard methods. Moreover, this electrochemical method is simple, cost-effective, convenient, sensitive, and holds great potential toward apoptosis evaluation, therapeutic effect assessment and deeper cellular biological studies. •A peptide-based electrochemical method for apoptosis evaluation is developed.•Interfacial charge transfer resistance can be used to assay captured apoptotic cells.•A strategy to assemble apoptotic cells on the solid surface is described.•Peptide-based recognition process offers merits over annexin V-based assays.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.06.035