Comparative evaluation of ELISA kit and HPLC DAD for the determination of chlorpyrifos ethyl residues in water and sediments

Enzyme linked immunosorbent assay (ELISA) kit is a versatile, cheap and relatively available tool that can be used in remote areas. In this study, performance of ELISA kit was evaluated in terms of accuracy, recovery, precision, sensitivity, cross reactivity and matrix interference for pesticide res...

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Bibliographic Details
Published in:Talanta (Oxford) 2013-12, Vol.117, p.250-257
Main Authors: Otieno, P.O., Owuor, P.O., Lalah, J.O., Pfister, G., Schramm, K.-W.
Format: Article
Language:English
Subjects:
Chlorpyrifos
Chlorpyrifos - analogs & derivatives
Chlorpyrifos - analysis
Chlorpyrifos ethyl
Chromatography, High Pressure Liquid
confidence interval
cross reaction
detection limit
Developing countries
ELISA
environmental assessment
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Geologic Sediments - chemistry
high performance liquid chromatography
HPLC
Humans
Kenya
lakes
Lakes - chemistry
Limit of Detection
Mathematical analysis
monitoring
pesticide residues
Pesticide Residues - analysis
Recovery
Reproducibility of Results
Residues
sampling
Sediment
Sediments
Validation
Water Pollutants, Chemical - analysis
Water quality
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Summary:Enzyme linked immunosorbent assay (ELISA) kit is a versatile, cheap and relatively available tool that can be used in remote areas. In this study, performance of ELISA kit was evaluated in terms of accuracy, recovery, precision, sensitivity, cross reactivity and matrix interference for pesticide residue determination in water and sediment samples. This method was compared with high performance liquid chromatography (HPLC) which is not a commonly available analytical technique for chlorpyrifos ethyl residue analysis in developing countries. The ELISA kit had limits of detection (LOD) of 0.37µgL−1 and 0.42µgKg−1 dry weight (dw), for chlorpyrifos ethyl in water and sediment samples, respectively using deionized water and a control sediment sample. Mean percentage recoveries and coefficients of variation (CV) for ELISA kit varied from 96.0±5.8% to 108.0±3.4% for water and sediment samples. Comparison between ELISA and HPLC analysis results using water and sediment samples from Lake Naivasha showed no significant difference in results (p≤0.05). Strong correlations (r2=0.9878 water samples and r1=0.9670, p
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2013.09.014