Evaluating the potential use of myxomycetes as a source of lipids for biodiesel production

► Procedure to grow myxomycete plasmodia without needing to add other living microbes. ► Procedure for analyzing lipid content and composition of myxomycete plasmodia. ► First report of using lipids derived from myxomycetes for biodiesel production. The myxomycetes are a group of primitive phagotrop...

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Bibliographic Details
Published in:Bioresource technology 2012-11, Vol.123, p.386-389
Main Authors: Tran, Hanh T.M., Stephenson, Steven L., Chen, Zhaorigetu, Pollock, Erik D., Goggin, Fiona L.
Format: Article
Language:English
Subjects:
Biodiesel
Biofuels - analysis
Biofuels - microbiology
Biomass
biomass production
Chondromyces
Diglycerides
Esters
eukaryotic cells
Fatty acids
gas chromatography
Lipids
Lipids - biosynthesis
mass spectrometry
Monoglycerides
Myxomycetes
Myxomycetes - growth & development
Myxomycetes - metabolism
phospholipids
Physarum polycephalum
Plasmodium
Species Specificity
Spectrometry
Spores, Protozoan - metabolism
thin layer chromatography
Time Factors
triacylglycerols
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Summary:► Procedure to grow myxomycete plasmodia without needing to add other living microbes. ► Procedure for analyzing lipid content and composition of myxomycete plasmodia. ► First report of using lipids derived from myxomycetes for biodiesel production. The myxomycetes are a group of primitive phagotrophic eukaryotes characterized by a distinctive plasmodial stage that is well known for its rapid growth rate. In the present study, biomass and lipid production of several different species of myxomycetes were investigated. Physarum polycephalum was found to produce the highest amounts of both dry biomass (1.30g), and lipid (0.143g) per 20mL medium (equal to 65.0g biomass and 7.15g lipid per one liter of medium). Analysis of P. polycephalum lipids by thin layer chromatography (TLC) and fatty acid methyl esters (FAMES) by gas chromatography–mass spectrometry (GC–MS) techniques showed that the major lipid type is triglyceride (95.5%), followed by phospholipids (2.6%); diglyceride (0.92%) and monoglyceride (0.92%). Myxomycete lipids consist of three dominant fatty acids: oleic (20%), linoleic (33%), and palmitoleic (17%). These results suggest that P. polycephalum has considerable potential as a source of lipids for biodiesel production.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.07.043