Huntingtin Aggregation Monitored by Dynamic Light Scattering

An initial stage of fibrillogenesis in solutions of glutathione S-transferase-huntingtin (GST-HD) fusion proteins has been studied by using dynamic light scattering. Two GST-HD systems with poly-L-glutamine (polyGln) extensions of different lengths (20 and 51 residues) have been examined. For both s...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-05, Vol.95 (11), p.6118-6121
Main Authors: Georgalis, Yannis, Starikov, E. B., Hollenbach, Birgit, Lurz, Rudi, Scherzinger, Eberhard, Saenger, Wolfram, Lehrach, Hans, Wanker, Erich E.
Format: Article
Language:English
Subjects:
Aggregation
Alzheimers disease
Biological Sciences
Diffusion coefficient
Exons
Glutathione Transferase - chemistry
Glutathione Transferase - genetics
Glutathione Transferase - metabolism
Humans
Huntingtin Protein
Huntington disease
Huntington Disease - metabolism
Kinetics
Light
Light scattering
Macromolecules
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurological disorders
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Physics
Protein Conformation
Proteins
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - metabolism
Solar fibrils
Spectrum Analysis
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Summary:An initial stage of fibrillogenesis in solutions of glutathione S-transferase-huntingtin (GST-HD) fusion proteins has been studied by using dynamic light scattering. Two GST-HD systems with poly-L-glutamine (polyGln) extensions of different lengths (20 and 51 residues) have been examined. For both systems, kinetics of z-average translation diffusion coefficients (Dapp) and their angular dependence have been obtained. Our data reveal that aggregation does occur in both GST-HD51 and GST-HD20 solutions, but that it is much more pronounced in the former. Thus, our approach provides a powerful tool for the quantitative assay of GST-HD fibrillogenesis in vitro.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.11.6118