Screening of complex fucoidans from four brown algae species as procoagulant agents

•Selected fucoidan species normalize thrombin generation of hemophilic plasma.•Fucoidans differ in undesired contact activation or in content of contaminants.•A screening strategy for natural fucoidan extracts is provided.•Analytical tools for characterizing complex fucoidan preparations were develo...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate polymers 2015-01, Vol.115, p.677-685
Main Authors: Zhang, Zhenqing, Till, Susanne, Knappe, Sabine, Quinn, Catherine, Catarello, James, Ray, G. Joseph, Scheiflinger, Friedrich, Szabo, Christina M., Dockal, Michael
Format: Article
Language:English
Subjects:
Alginates - analysis
Anticoagulants - chemistry
Anticoagulants - pharmacology
Blood Coagulation - drug effects
Coagulants - chemistry
Coagulants - pharmacology
Fucoidan
Hemophilia
Hemostasis
Humans
Lipoproteins - antagonists & inhibitors
Monosaccharides - analysis
NMR
Partial Thromboplastin Time
Phaeophyceae
Polysaccharides - chemistry
Polysaccharides - pharmacology
Screening
Structure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Selected fucoidan species normalize thrombin generation of hemophilic plasma.•Fucoidans differ in undesired contact activation or in content of contaminants.•A screening strategy for natural fucoidan extracts is provided.•Analytical tools for characterizing complex fucoidan preparations were developed.•Fucus vesiculosus fucoidan was identified as the most suitable procoagulant agent. Fucoidans are complex sulfated polysaccharides extracted from brown algae. Depending on the concentration, they have been shown to stimulate and inhibit blood coagulation in vitro. Promotion of coagulation is mediated by blocking tissue factor pathway inhibitor (TFPI). We screened fucoidan extracts from four brown algae species in vitro with respect to their potential to improve coagulation in bleeding disorders. The fucoidans’ pro- and anticoagulant activities were assessed by global hemostatic and standard clotting assays. Results showed that fucoidans improved coagulation parameters. Some fucoidans also activated the contact pathway of coagulation, an undesired property reported for sulfated glycosaminoglycans. Chemical evaluation of fucoidans’ complex and variable structure included molecular weight (Mw), polydispersity (polyD), structural heterogeneity, and organic and inorganic impurities. Herewith, we describe a screening strategy that facilitates the identification of crude fucoidan extracts with desired biological and structural properties for improvement of compromised coagulation like in hemophilia.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2014.09.001