Cytochrome P450 genes induced by bispyribac‐sodium treatment in a multiple‐herbicide‐resistant biotype of Echinochloa phyllopogon

BACKGROUND: Incremental herbicide metabolism by cytochrome P450 monooxygenases (P450s) has been proposed as the basis for resistance to bispyribac‐sodium (bispyribac) in a multiple‐herbicide‐resistant biotype of Echinochloa phyllopogon. Upon exposure to bispyribac, strong induction of bispyribac‐met...

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Bibliographic Details
Published in:Pest management science 2014-04, Vol.70 (4), p.549-558
Main Authors: Iwakami, Satoshi, Uchino, Akira, Kataoka, Yukiko, Shibaike, Hiroyuki, Watanabe, Hiroaki, Inamura, Tatsuya
Format: Article
Language:English
Subjects:
ALS inhibitor
Amino Acid Sequence
amino acid sequences
Benzoates - metabolism
Benzoates - toxicity
Biological and medical sciences
Crop science
cytochrome P-450
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Echinochloa
Echinochloa - genetics
Echinochloa - metabolism
Echinochloa oryzoides
Fundamental and applied biological sciences. Psychology
Gene Expression
gene expression regulation
Genes
herbicide metabolism
herbicide resistance
Herbicide Resistance - genetics
herbicide stress response
Herbicides
late watergrass
metabolism
non-target-site-based resistance
Parasitic plants. Weeds
Pest control
Phytopathology. Animal pests. Plant and forest protection
Plant resistance
polymerase chain reaction
Polymorphism, Genetic
Pyrimidines - metabolism
Pyrimidines - toxicity
seedlings
Weeds
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Summary:BACKGROUND: Incremental herbicide metabolism by cytochrome P450 monooxygenases (P450s) has been proposed as the basis for resistance to bispyribac‐sodium (bispyribac) in a multiple‐herbicide‐resistant biotype of Echinochloa phyllopogon. Upon exposure to bispyribac, strong induction of bispyribac‐metabolising P450 activity has been reported in the resistant line, indicating that P450s induced by bispyribac are involved in the bispyribac resistance. RESULTS: A polymerase chain reaction (PCR)‐based cloning strategy was used to isolate 39 putative P450 genes from the bispyribac‐resistant line of E. phyllopogon. Expression analysis by real‐time PCR revealed that seven of the isolated genes were upregulated in response to bispyribac treatment of seedlings at the three‐leaf stage. The transcript levels and protein sequences of the seven genes were compared between the bispyribac‐resistant line and a susceptible line. CYP71AK2 and CYP72A254 were transcribed prominently in the bispyribac‐resistant line. Amino acid polymorphisms were found in three genes, including CYP72A254. CONCLUSION: Upregulated expression of these genes is consistent with the inducible herbicide‐metabolising P450 activity under bispyribac stress that was reported in a previous study. This is the first study to compare P450 genes in arable weed species in order to elucidate the mechanism for P450‐mediated herbicide resistance. © 2013 Society of Chemical Industry
ISSN:1526-498X
1526-4998
DOI:10.1002/ps.3572