Gastric juice long noncoding RNA used as a tumor marker for screening gastric cancer

BACKGROUND Long noncoding RNAs (lncRNAs) play a crucial role in tumorigenesis. However, the value of lncRNAs in the diagnosis of gastric cancer remains unknown. To identify whether lncRNA‐AA174084 is a potential marker for the early diagnosis of gastric cancer (GC), the authors investigated its leve...

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Published in:Cancer 2014-11, Vol.120 (21), p.3320-3328
Main Authors: Shao, Yongfu, Ye, Meng, Jiang, Xiaoming, Sun, Weiliang, Ding, Xiaoyun, Liu, Zhong, Ye, Guoliang, Zhang, Xinjun, Xiao, Bingxiu, Guo, Junming
Format: Article
Language:English
Subjects:
AA174084
Adult
Aged
Biological and medical sciences
biomarker
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Early Detection of Cancer
Female
gastric cancer
Gastric Juice
Gastroenterology. Liver. Pancreas. Abdomen
Humans
lncRNA
long noncoding RNA
Lymphatic Metastasis - genetics
Male
Medical sciences
Middle Aged
molecular diagnosis
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Neoplasm Staging
RNA, Long Noncoding - biosynthesis
RNA, Long Noncoding - blood
RNA, Long Noncoding - genetics
Stomach Neoplasms - blood
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:BACKGROUND Long noncoding RNAs (lncRNAs) play a crucial role in tumorigenesis. However, the value of lncRNAs in the diagnosis of gastric cancer remains unknown. To identify whether lncRNA‐AA174084 is a potential marker for the early diagnosis of gastric cancer (GC), the authors investigated its levels in tissues, blood, and gastric juices from patients with various stage of gastric tumorigenesis. METHODS Total RNA in 860 specimens from patients and healthy controls was extracted. Levels of AA174084 in 134 paired GC tissues, 127 gastric mucosal tissues, 335 plasma samples, and 130 gastric juice samples at each stage of gastric tumorigenesis were measured using real‐time reverse transcriptase‐polymerase chain reaction analysis. The potential association between AA174084 levels and patients' clinicopathologic features were analyzed. A receiver operating characteristic (ROC) curve was constructed for differentiating GC patients from controls. RESULTS Expression levels of AA174084 were down‐regulated significantly in 95 of 134 GC tissues (71%) compared with the levels in paired, adjacent, normal tissues (P < .001). AA174084 levels had significant, negative correlations with age (P = .031), Borrmann type (P = .016), and perineural invasion (P = .032). Plasma AA174084 levels in patients with GC dropped markedly on day 15 after surgery compared with preoperative levels (P < .001) and were associated with invasion (P = .049) and lymphatic metastasis (P = .042). AA174084 levels in gastric juice from patients with GC were significantly higher than the levels in normal mucosa or in patients with minimal gastritis, gastric ulcers, and atrophic gastritis (P < .001). The area under ROC was up to 0.848 (P < .001). CONCLUSIONS AA174084 may have potential as marker for the early diagnosis of GC. Cancer 2014;120:3320–3328. © 2014 American Cancer Society. Long noncoding RNAs (lncRNAs) stably exist in gastric juice and can be detected using real‐time quantitative reverse transcription‐polymerase chain reaction analysis. Some lncRNAs, such as AA174084 in gastric juice, can be used as biomarkers for the detection of gastric cancer with satisfactory specificity and sensitivity.
ISSN:0008-543X
1097-0142
DOI:10.1002/cncr.28882