Effects of lung exposure to carbon nanotubes on female fertility and pregnancy. A study in mice

•In dams, lung and liver bore evidence of MWCNT exposure 4 months after exposure.•Preconceptional exposure to MWCNT did not seem to affect pregnancy and offspring.•Delivery of a first litter was slightly delayed in female mice instilled MWCNT prior to cohabitation with a male. We studied the effects...

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Published in:	Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2013-11, Vol.41, p.86-97
Main Authors:	Hougaard, Karin S., Jackson, Petra, Kyjovska, Zdenka O., Birkedal, Renie K., De Temmerman, Pieter-Jan, Brunelli, Andrea, Verleysen, Eveline, Madsen, Anne Mette, Saber, Anne T., Pojana, Giulio, Mast, Jan, Marcomini, Antonio, Jensen, Keld A., Wallin, Håkan, Szarek, Józef, Mortensen, Alicja, Vogel, Ulla
Format:	Article
Language:	eng
Subjects:	Animals Bronchoalveolar Lavage Fluid - cytology Developmental toxicity Female Fertility - drug effects Liver - drug effects Liver - pathology Lung - drug effects Lung - pathology Lung and liver pathology Male Mice Mice, Inbred C57BL Motor Activity - drug effects Multiwalled carbon nanotubes Nanotoxicology Nanotubes, Carbon - toxicity Pneumonia - chemically induced Pneumonia - pathology Pregnancy Reflex, Startle - drug effects Reproductive toxicity Spermatogenesis - drug effects
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Summary:	•In dams, lung and liver bore evidence of MWCNT exposure 4 months after exposure.•Preconceptional exposure to MWCNT did not seem to affect pregnancy and offspring.•Delivery of a first litter was slightly delayed in female mice instilled MWCNT prior to cohabitation with a male. We studied the effects of preconceptional exposure to multiwalled carbon nanotubes (MWCNTs): mature, female C57BL/6J mice were intratracheally instilled with 67µg NM-400 MWCNT, and the following day co-housed with mature males, in breeding pairs. Time to delivery of the first litter, litter parameters, maternal inflammation and histopathology of lung and liver were recorded. In male offspring, locomotor activity, startle response, and daily sperm production (DSP) were assessed. In the dams, lung and liver bore evidence of MWCNT exposure when assessed 6 weeks and 4 months after exposure. A short delay in the delivery of the first litter was observed in exposed females. Litter parameters, behavior and DSP were similar in control and exposed groups. In conclusion, instillation of a single dose of MWCNT induced long lasting pathological changes in dam lung and liver. Theoretically, lung inflammation due to particle exposure could interfere with female reproductive parameters. Whether the observed lag in delivery of a first litter was in fact caused by exposure to MWCNT should be addressed in a study designed specifically to elucidate effects on the early processes involved in establishment of pregnancy. Exposure was not associated with changes in the assessed gestational or offspring parameters.
ISSN:	0890-6238 1873-1708