A wheat lipid transfer protein 3 could enhance the basal thermotolerance and oxidative stress resistance of Arabidopsis

Wheat (Triticum aestivum L.) is one of the major grain crops, and heat stress adversely affects wheat production in many regions of the world. Previously, we found a heat-responsive gene named Lipid Transfer Protein 3 (TaLTP3) in wheat. TaLTP3 was deduced to be regulated by cold, ABA, MeJA, Auxin an...

Full description

Saved in:
Bibliographic Details
Published in:Gene 2014-10, Vol.550 (1), p.18-26
Main Authors: Wang, Fei, Zang, Xin-shan, Kabir, Muhammad Rezaul, Liu, Ke-lu, Liu, Zhen-shan, Ni, Zhong-fu, Yao, Ying-yin, Hu, Zhao-rong, Sun, Qi-xin, Peng, Hui-ru
Format: Article
Language:English
Subjects:
Abscisic Acid - pharmacology
Adaptation, Physiological - drug effects
Adaptation, Physiological - genetics
Adaptation, Physiological - physiology
Amino Acid Sequence
Antioxidant
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis - physiology
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell Membrane - metabolism
Cytoplasm - metabolism
Gene Expression Regulation, Plant - drug effects
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hot Temperature
Hydrogen Peroxide - metabolism
Microscopy, Confocal
Molecular Sequence Data
Nicotiana - cytology
Nicotiana - genetics
Nicotiana - metabolism
Oxidative Stress
Plant Epidermis - cytology
Plant Epidermis - metabolism
Plant Growth Regulators - pharmacology
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Seedlings - genetics
Seedlings - metabolism
Seedlings - physiology
Sequence Homology, Amino Acid
Thermotolerance
Time Factors
Triticum - genetics
Triticum - metabolism
Triticum - physiology
Triticum aestivum
Wheat lipid transfer protein 3
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Wheat (Triticum aestivum L.) is one of the major grain crops, and heat stress adversely affects wheat production in many regions of the world. Previously, we found a heat-responsive gene named Lipid Transfer Protein 3 (TaLTP3) in wheat. TaLTP3 was deduced to be regulated by cold, ABA, MeJA, Auxin and oxidative stress according to cis-acting motifs in its promoter sequences. In this study, we show that TaLTP3 is responsive to prolonged water deficit, salt or ABA treatment in wheat seedlings. Also, TaLTP3 accumulation was observed after the plant suffered from heat stress both at the seedling and the grain-filling stages. TaLTP3 protein was localized in the cell membrane and cytoplasm of tobacco epidermal cells. Overexpression of TaLTP3 in yeast imparted tolerance to heat stress compared to cells expressing the vector alone. Most importantly, transgenic Arabidopsis plants engineered to overexpress TaLTP3 showed higher thermotolerance than control plants at the seedling stage. Further investigation indicated that transgenic lines decreased H2O2 accumulation and membrane injury under heat stress. Taken together, our results demonstrate that TaLTP3 confers heat stress tolerance possibly through reactive oxygen species (ROS) scavenging. •TaLTP3 could respond to drought, salt and heat stress.•Overexpression of TaLTP3 conferred thermotolerance to yeast and Arabidopsis.•TaLTP3 overexpressed Arabidopsis accumulates less ROS after heat stress.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2014.08.007