Increased trophoblast expression of NFAT5/TonEBP in pre-eclamptic placentas and hyperosmolar-treated BeWo cells

Abstract Objectives To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase...

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Published in:European journal of obstetrics & gynecology and reproductive biology 2014-12, Vol.183, p.37-43
Main Authors: Lee, G.T, Price, M.D, Mejia, C.A, Galan, H.L, Arroyo, J.A
Format: Article
Language:English
Subjects:
Aldehyde Reductase - metabolism
Case-Control Studies
Cells, Cultured
Choriocarcinoma - metabolism
Choriocarcinoma - pathology
Extracellular Signal-Regulated MAP Kinases - metabolism
Female
Heat-Shock Proteins - metabolism
Humans
In Vitro Techniques
Inositol
Inositol - metabolism
NFAT5/TonEBP
Obstetrics and Gynecology
Osmolar Concentration
Placenta - drug effects
Placenta - metabolism
Placenta - pathology
Pre-eclampsia
Pre-Eclampsia - metabolism
Pre-Eclampsia - pathology
Pregnancy
Saline Solution, Hypertonic - pharmacology
Sorbitol
Sorbitol - metabolism
Symporters - metabolism
Transcription Factors - metabolism
Trophoblast
Trophoblasts - drug effects
Trophoblasts - metabolism
Trophoblasts - pathology
Uterine Neoplasms - metabolism
Uterine Neoplasms - pathology
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Summary:Abstract Objectives To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase] in placentas of pre-eclamptic (PE) patients and trophoblast BeWo cells subjected to hypertonic stress in vitro. Study design Control and PE placentas were collected. BeWo cells were cultured and subjected to a hyperosmolar solution for 4 h. Western blot analysis was performed on NFAT5, SLC5A3, aldose reductase and ERK proteins. High-performance liquid chromatography was used to determine the levels of inositol and sorbitol in cell lysates. Results Compared with control placentas, PE placentas showed higher levels of inositol and NFAT5, and lower levels of SLC5A3. Treated BeWo cells showed higher levels of inositol, sorbitol, NFAT5 total protein, SLC5A3 and aldose reductase, and increased ERK activation compared with control BeWo cells. Conclusions Hyperosmolar conditions increase the expression of NFAT5 in PE placentas and BeWo cells, and may account for the increased osmolyte levels. NFAT5 may accomplish this through aldose reductase and SLC5A3 in trophoblast cells.
ISSN:0301-2115
1872-7654
DOI:10.1016/j.ejogrb.2014.10.002