The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses

In this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacteria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions of heavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. B...

Full description

Saved in:
Bibliographic Details
Published in:Microbiological research 2015-01, Vol.170, p.147-156
Main Authors: Chudobova, Dagmar, Dostalova, Simona, Ruttkay-Nedecky, Branislav, Guran, Roman, Rodrigo, Miguel Angel Merlos, Tmejova, Katerina, Krizkova, Sona, Zitka, Ondrej, Adam, Vojtech, Kizek, Rene
Format: Article
Language:English
Subjects:
Heavy metals
Ions - metabolism
Ions - pharmacology
Mass Spectrometry
Metabolome
Metabolomics - methods
Metallothionein - chemistry
Metallothionein - metabolism
Metals - metabolism
Metals - pharmacology
Microbial Viability - drug effects
Resistance
RNA, Ribosomal - genetics
S. aureus
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Staphylococcus aureus - growth & development
Staphylococcus aureus - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacteria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions of heavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochemical changes of resistant strains in comparison with the non-resistant control strain of S. aureus were observed by microbiological (measuring - growth curves and inhibition zones) and spectrophotometric methods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phosphatase, γ-glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysis of the samples (changes in S. aureus protein composition) and for the identification of the strains database MALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition, were observed in resistant strains compared to non-resistant control strain. Our results describe the possible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoring of changes in genetic information caused by the forming of resistance to heavy metals.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2014.08.003