Serum Metabolomics Study and Eicosanoid Analysis of Childhood Atopic Dermatitis Based on Liquid Chromatography–Mass Spectrometry

Atopic dermatitis (AD) is the most common inflammatory skin disease in children. In the study, ultra high performance liquid chromatography–mass spectrometry was used to investigate serum metabolic abnormalities of AD children. Two batch fasting sera were collected from AD children and healthy contr...

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Bibliographic Details
Published in:Journal of proteome research 2014-12, Vol.13 (12), p.5715-5723
Main Authors: Huang, Yan, Chen, Guoyou, Liu, Xinyu, Shao, Yaping, Gao, Peng, Xin, Chenchen, Cui, Zhenze, Zhao, Xinjie, Xu, Guowang
Format: Article
Language:English
Subjects:
Bile Acids and Salts - blood
Biomarkers - blood
Carnitine - blood
Child, Preschool
Chromatography, Liquid - methods
Dermatitis, Atopic - blood
Dermatitis, Atopic - diagnosis
Dermatitis, Atopic - metabolism
Eicosanoids - blood
Eicosanoids - metabolism
Fatty Acids, Nonesterified - blood
Female
Humans
Immunoglobulin E - blood
Infant
Lactic Acid - blood
Leukotriene B4 - blood
Male
Mass Spectrometry - methods
Metabolome
Metabolomics - methods
Prostaglandins - blood
Reproducibility of Results
ROC Curve
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Summary:Atopic dermatitis (AD) is the most common inflammatory skin disease in children. In the study, ultra high performance liquid chromatography–mass spectrometry was used to investigate serum metabolic abnormalities of AD children. Two batch fasting sera were collected from AD children and healthy control; one of them was for nontargeted metabolomics analysis, the other for targeted eicosanoids analysis. AD children were divided into high immunoglobulin E (IgE) group and normal IgE group. On the basis of the two analysis approaches, it was found that the differential metabolites of AD, leukotriene B4, prostaglandins, conjugated bile acids, etc., were associated with inflammatory response and bile acids metabolism. Carnitines, free fatty acids, lactic acid, etc., increased in the AD group with high IgE, which revealed energy metabolism disorder. Amino acid metabolic abnormalities and increased levels of Cytochrome P450 epoxygenase metabolites were found in the AD group with normal IgE. The results provided a new perspective to understand the mechanism and find potential biomarkers of AD and may provide a new reference for personalized treatment.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr5007069