Colorimetric method for a rapid detection of oxygenated aromatic biotransformation products

The quantitative production of the oxygenated products from the biotransformation of aromatic substrates can be detected using a very simple and rapid colorimetric test. The method is based on Gibbs' reagent (2,6-dichloroquinone-4-chloroimide) and has been developed for routine spectrophotometr...

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Bibliographic Details
Published in:Biotechnology techniques 1997, Vol.11 (8), p.585-587
Main Authors: QUINTANA, M. G, DIDION, C, DALTON, H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Various methods and equipments
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Summary:The quantitative production of the oxygenated products from the biotransformation of aromatic substrates can be detected using a very simple and rapid colorimetric test. The method is based on Gibbs' reagent (2,6-dichloroquinone-4-chloroimide) and has been developed for routine spectrophotometric or microtitre plate assay allowing the detection of products with a sensitivity as low as 5 mu M.
ISSN:0951-208X
1573-6784
DOI:10.1023/a:1018499024466