Abscisic acid signal transduction in the barley aleurone is mediated by phospholipase D activity

The plant hormones abscisic acid (ABA) and gibberellic acid (GA) are important regulators of the dormancy and germination of seeds. In cereals, GA enhances the synthesis and secretion of enzymes (principally alpha-amylases) in the aleurone cells of the endosperm, which then mobilize the storage rese...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-03, Vol.95 (5), p.2697-2702
Main Authors: Ritchie, S. (Pennsylvania State University, University, PA.), Gilroy, S
Format: Article
Language:English
Subjects:
1-Butanol - pharmacology
ABA
Abscisic Acid - pharmacology
ACIDO GIBERELICO
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Alcohols
ALEURONE CELLS
ALEURONE LAYER
ALFA AMILASA
ALPHA AMYLASE
Barley
Biological Sciences
BUTANOL
CAPA DE ALEURONA
CHEMICAL COMPOSITION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
COUCHE A ALEURONE
Diacylglycerol Kinase - metabolism
DIACYLGLYCEROLS
Diglycerides - metabolism
Enzyme Activation
ENZYME INHIBITORS
Enzyme Inhibitors - pharmacology
Enzymes
ENZYMIC ACTIVITY
ESTERASAS
ESTERASE
ESTERASES
ESTIMULO
FOSFOLIPIDOS
GIBBERELLINE
Gibberellins - pharmacology
GLICERIDOS
GLYCERIDE
GLYCERIDES
Hordeum - physiology
HORDEUM VULGARE
INHIBICION
INHIBIDORES DE ENZIMAS
INHIBITEUR D'ENZYME
INHIBITION
Kinetics
Lipids
PHOSPHATIDE
PHOSPHATIDIC ACIDS
Phosphatidic Acids - metabolism
Phospholipase D - antagonists & inhibitors
Phospholipase D - metabolism
PHOSPHOLIPIDS
PHOSPHORIC DIESTER HYDROLASES
Plant cells
PLANT PROTEINS
PROTEIN SYNTHESIS
PROTEINAS
PROTEINE
PROTEINS
PROTOPLASTE
PROTOPLASTOS
PROTOPLASTS
Protoplasts - drug effects
Protoplasts - physiology
RAB PROTEINS
Secretion
Signal transduction
Signal Transduction - drug effects
Signal Transduction - physiology
SINTESIS DE PROTEINAS
Space life sciences
STIMULI
STIMULUS
SYNTHESE PROTEIQUE
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Summary:The plant hormones abscisic acid (ABA) and gibberellic acid (GA) are important regulators of the dormancy and germination of seeds. In cereals, GA enhances the synthesis and secretion of enzymes (principally alpha-amylases) in the aleurone cells of the endosperm, which then mobilize the storage reserves that fuel germination. ABA inhibits this enhanced secretory activity and delays germination. Despite the central role of ABA in regulating germination, the signal transduction events leading to altered gene expression and cellular activity are essentially unknown. We report that the application of ABA to aleurone protoplasts increased the activity of the enzyme phospholipase D (PLD) 10 min after treatment. The product of PLD activity, phosphatidic acid (PPA), also increased transiently at this time. The application of PPA to aleurone protoplasts led to an ABA-like inhibition of alpha-amylase production, and induction of the ABA up-regulated proteins ASI (amylase subtilisin inhibitor) and RAB (responsive to ABA). Inhibition of PLD activity by 0.1% 1-butanol during the initial 20 min of ABA treatment resulted in inhibition of ABA-regulated processes. This inhibition coincided with the timing of PLD activation by ABA and was overcome by simultaneous addition of PPA. These results suggest that ABA activates the enzyme PLD to produce PPA that is involved in triggering the subsequent ABA responses of the aleurone cell
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.5.2697