A novel technique for entrapment of hybridoma cells in synthetic thermally reversible polymers

A new technique for entrapment of cells in thermally reversible polymer (poly-N-vinylcaprolactam - PVCl) has been developed. Using stabilizers, such as ovalbumin, sodium carboxymethylcellulose or egg powder were necessary to give structures that were stable upon stirring carriers. Two hybridoma line...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology techniques 1991-05, Vol.5 (3), p.223-226
Main Authors: MARKVICHEVA, E. A, KUZ'KINA, I. F, PASHKIN, I. I, PLECHKO, T. N, KIRSH, YU. E, ZUBOV, V. P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
hybridoma
immobilization
Immobilization of organelles and whole cells
Immobilization techniques
immobilized cells
Methods. Procedures. Technologies
monoclonal antibodies
poly-N-vinylcaprolactam
polymers
production
supports
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new technique for entrapment of cells in thermally reversible polymer (poly-N-vinylcaprolactam - PVCl) has been developed. Using stabilizers, such as ovalbumin, sodium carboxymethylcellulose or egg powder were necessary to give structures that were stable upon stirring carriers. Two hybridoma lines, in particular, 3B4 and 1A10 cells were entrapped in gel and produced monoclonal antibodies IgG and IgM classes, respectively, against Arabis mosaic virus.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00152786