O2 reduction by photosystem I involves phylloquinone under steady-state illumination

•Rate of O2 reduction by PSI increases with increasing light intensity.•Quinone nature at the A1-site affects O2 reduction by PSI.•Phyllosemiquinone at the A1-site reduces O2. O2 reduction was investigated in photosystem I (PSI) complexes isolated from cyanobacteria Synechocystis sp. PCC 6803 wild t...

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Bibliographic Details
Published in:FEBS letters 2014-11, Vol.588 (23), p.4364-4368
Main Authors: Kozuleva, Marina A., Petrova, Anastasia A., Mamedov, Mahir D., Semenov, Alexey Yu, Ivanov, Boris N.
Format: Article
Language:English
Subjects:
1-hydroxy-4-isobutyramido-2,2,6,6-tetramethylpiperidinium
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Binding Sites
Coenzymes - chemistry
Coenzymes - metabolism
dimethylformamide
DMF
Electron transfer
electron transfer chain
Electron Transport - radiation effects
ETC
ferredoxin
Gene Knockout Techniques
Light
methyl viologen
Models, Molecular
Molecular Conformation
Mutation
N,N,N′,N′-tetramethyl-p-phenylenediamine
Oxygen - metabolism
Oxygen reduction
Photosynthesis
Photosystem I
Photosystem I Protein Complex - metabolism
PhQ and PhQ
Phylloquinone
phylloquinone and phyllosemiquinone
Plastoquinone
plastoquinone and plastosemiquinone
PQ and PQ
PS I
rates of electron transfer as such and to O2 and MV, respectively
reactive oxygen species
ROS
Synechocystis - enzymology
Synechocystis - genetics
Synechocystis - metabolism
Synechocystis - radiation effects
TMPD
TMT-H
V ET, VO2ET and V ET MV
Vitamin K 1 - metabolism
wild type
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Summary:•Rate of O2 reduction by PSI increases with increasing light intensity.•Quinone nature at the A1-site affects O2 reduction by PSI.•Phyllosemiquinone at the A1-site reduces O2. O2 reduction was investigated in photosystem I (PSI) complexes isolated from cyanobacteria Synechocystis sp. PCC 6803 wild type (WT) and menB mutant strain, which is unable to synthesize phylloquinone and contains plastoquinone at the quinone-binding site A1. PSI complexes from WT and menB mutant exhibited different dependencies of O2 reduction on light intensity, namely, the values of O2 reduction rate in WT did not reach saturation at high intensities, in contrast to the values in menB mutant. The obtained results suggest the immediate phylloquinone involvement in the light-induced O2 reduction by PSI.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2014.10.003