Nitric oxide synthase inhibitor influences prostaglandin and interleukin-1 production in experimental arthritic joints

To assess involvement of nitric oxide (NO) in the increase in eicosanoid and interleukin- 1 (IL-1) levels in the synovial fluid during antigen-induced arthritis (AIA) in rabbits treated with a competitive inhibitor of NO synthesis. Thirteen New Zealand White rabbits were sensitized with 5 mg of meth...

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Bibliographic Details
Published in:Inflammation research 1997-02, Vol.46 (2), p.72-77
Main Authors: de Mello, S B, Novaes, G S, Laurindo, I M, Muscará, M N, Maciel, F M, Cossermelli, W
Format: Article
Language:English
Subjects:
Animals
Arthritis, Experimental - drug therapy
Arthritis, Experimental - metabolism
Arthritis, Experimental - pathology
Capillary Permeability - drug effects
Capillary Permeability - immunology
Chemotaxis, Leukocyte - drug effects
Chemotaxis, Leukocyte - immunology
Dinoprostone - adverse effects
Dinoprostone - biosynthesis
Dinoprostone - chemistry
Dinoprostone - metabolism
Disease Models, Animal
Eicosanoids - chemistry
Interleukin-1 - biosynthesis
Interleukin-1 - chemistry
Interleukin-1 - metabolism
Knee Joint - drug effects
Knee Joint - pathology
Male
Nitrates - analysis
Nitric Oxide - adverse effects
Nitric Oxide - chemistry
Nitric Oxide - metabolism
Nitric Oxide Synthase - adverse effects
Nitric Oxide Synthase - antagonists & inhibitors
Nitric Oxide Synthase - metabolism
Nitrites - analysis
Rabbits
Synovial Fluid - chemistry
Synovial Membrane - pathology
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Summary:To assess involvement of nitric oxide (NO) in the increase in eicosanoid and interleukin- 1 (IL-1) levels in the synovial fluid during antigen-induced arthritis (AIA) in rabbits treated with a competitive inhibitor of NO synthesis. Thirteen New Zealand White rabbits were sensitized with 5 mg of methylated bovine serum albumin (mBSA). Arthritis was induced in the knee joint by injecting 0.5 ml of a sterile solution of mBSA (2 mg/ml) into the intra-articular cavity. Prior to the induction of arthritis, the animals received N-Omega-Nitro-L-Arginine Methyl Ester (LNAME) or N-Omega-Nitro-D-Arginine Methyl Ester (DNAME) for 2 weeks, both at a dose of 20 mg/kg/day mixed with drinking water. Leukocyte efflux (total and differential white cell count), vascular permeability (Evans's blue method), synovial PMN cell infiltrate, and total nitrite (NO2.)/nitrate (NO3.) (HPLC), PGE2, TxB2, LTB4 (radioimmunoassay), and IL-1 beta (ELISA) levels were quantified in the synovial fluid. LNAME but not DNAME significantly suppressed leukocyte efflux and protein leakage into the articular cavity as well as synovial PMN cell infiltrate. Total NO2./NO3., PGE2 and IL-1 beta levels were significantly reduced in the synovial fluid of LNAME treated animals. TxB2 and LTB4 were not affected by LNAME treatment. These data clearly show NO involvement in the IL-1-induced PGE2 production in the synovial fluid of antigen-induced arthritis in rabbits.
ISSN:1023-3830
1420-908X
DOI:10.1007/s000110050086