Urinary zearalenone measured with ELISA as a biomarker of zearalenone exposure in pigs

The suitability to assess zearalenone (ZEA) exposure in pigs of a commercial ELISA kit for ZEA analysis in urine was tested. A daily dose of 0, 5, 10, 20 and 40 μg synthetic ZEA per kilogram BW was administered via the feed to four gilts per dose group, and after 3 and after 7 days of ZEA intake, ur...

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Bibliographic Details
Published in:Mycotoxin research 2014-11, Vol.30 (4), p.187-190
Main Authors: Gutzwiller, A, Gafner, J. L, Silacci, P
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomarkers
Biomarkers - analysis
Biomedical and Life Sciences
Chemistry/Food Science
creatinine
cross reaction
diet
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Feeds
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
gilts
Hogs
Life Sciences
Medical Microbiology
Medical sciences
Medicine/Public Health
Microbiology
Original Paper
Plant poisons toxicology
Swine
Toxicology
urinalysis
Urine
Urine - chemistry
zearalenone
Zearalenone - analysis
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Summary:The suitability to assess zearalenone (ZEA) exposure in pigs of a commercial ELISA kit for ZEA analysis in urine was tested. A daily dose of 0, 5, 10, 20 and 40 μg synthetic ZEA per kilogram BW was administered via the feed to four gilts per dose group, and after 3 and after 7 days of ZEA intake, urine samples were assayed with the ELISA which has a relative cross-reactivity of 42 % with α-zearalenol. The concentration of urinary ZEA equivalents (ZEA plus 42 % of α-zearalenol present) did not differ between day 4 and day 8 (P = 0.50) within each dose group. The urinary ZEA equivalent/creatinine ratio was tightly correlated with ZEA intake (r = 0.95). The urinary ZEA equivalent/creatinine values at 0 and 40 μg/kg BW were distinctly different from those of the intermediate dose levels, whereas there was some overlapping of the individual values at the dose levels 5, 10 and 20 μg/kg BW. The urinary ZEA equivalent/creatinine ratio can be used as a biomarker for ZEA exposure in pigs provided that urine samples of several animals receiving the same diet are assayed, either separately or after pooling.
ISSN:0178-7888
1867-1632
DOI:10.1007/s12550-014-0198-y