Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays

The feasibility of super-resolution microscopy has been investigated based on random localization of surface plasmon using blocked random nanodot arrays. The resolution is mainly determined by the size of localized fields in the range of 100-150 nm. The concept was validated by imaging FITC-conjugat...

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Bibliographic Details
Published in:Optics express 2014-11, Vol.22 (22), p.27695-27706
Main Authors: Oh, Youngjin, Son, Taehwang, Kim, Sook Young, Lee, Wonju, Yang, Heejin, Choi, Jong-Ryul, Shin, Jeon-Soo, Kim, Donghyun
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Animals
Cell Line
Image Processing, Computer-Assisted
Intracellular Space - metabolism
Mice
Microscopy, Fluorescence
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Numerical Analysis, Computer-Assisted
Surface Plasmon Resonance - methods
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Summary:The feasibility of super-resolution microscopy has been investigated based on random localization of surface plasmon using blocked random nanodot arrays. The resolution is mainly determined by the size of localized fields in the range of 100-150 nm. The concept was validated by imaging FITC-conjugated phalloidin that binds to cellular actin filaments. The experimental results confirm improved resolution in reconstructed images. Effect of far-field registration on image reconstruction was also analyzed. Correlation between reconstructed images was maintained to be above 81% after registration. Nanodot arrays are synthesized by temperature-annealing without sophisticated lithography and thus can be mass-produced in an extremely large substrate. The results suggest a super-resolution imaging technique that can be accessible and available in large amounts.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.22.027695