Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR)

Molecular examination of the ribosomal internal transcribed spacer (ITS) region in potato cyst nematodes (PCN) is described. The ITS was amplified and sequenced from a number of PCN collections. A low level of sequence variation was found between Globodera rostochiensis, G. pallida, and a Peruvian P...

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Bibliographic Details
Published in:New Zealand journal of crop and horticultural science 1997-06, Vol.25 (2), p.123-129
Main Authors: Bulman, S. R., Marshall, J. W.
Format: Article
Language:English
Subjects:
Globodera pallida
Globodera rostochiensis
microheterogeneity
PCN
PCR
ribosomal ITS
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Summary:Molecular examination of the ribosomal internal transcribed spacer (ITS) region in potato cyst nematodes (PCN) is described. The ITS was amplified and sequenced from a number of PCN collections. A low level of sequence variation was found between Globodera rostochiensis, G. pallida, and a Peruvian PCN collection, but no variation within Australasian collections of species was noted. Polymerase chain reaction (PCR) primers based upon the G. rostochiensis-G. pallida sequence differences were designed and successfully used to identify mixed PCN species in a single PCR reaction.
ISSN:0114-0671
0014-0671
1175-8783
DOI:10.1080/01140671.1997.9513998