Immortalization of normal human oral keratinocytes with type 16 human papillomavirus

Primary human oral keratinocytes were transformed by transfection with recombinant human papillomavirus type 16 (HPV-16)DNA, and two transformed cell lines named human oral keratinocytes-16A and -16B (HOK-16A and HOK-16B) were established. While normal cells and cells transfected with vector only ex...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1991-09, Vol.12 (9), p.1627-1631
Main Authors: Park, No-Hee, Min, Byung-Moo, Li, Sheng-lin, Huang, Min Zhong, Doniger, Jay
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Cell Transformation, Viral
DNA, Viral - genetics
General aspects (metabolism, cell proliferation, established cell line...)
Humans
Keratinocytes - pathology
Medical sciences
Mouth Mucosa - pathology
Nucleic Acid Hybridization
Papillomaviridae - isolation & purification
Plasmids
RNA, Viral - genetics
Tumor cell
Tumors
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Summary:Primary human oral keratinocytes were transformed by transfection with recombinant human papillomavirus type 16 (HPV-16)DNA, and two transformed cell lines named human oral keratinocytes-16A and -16B (HOK-16A and HOK-16B) were established. While normal cells and cells transfected with vector only exhibited a limited Wespan, the HOK-16A and HOK-16B lines demonstrated immortality and altered morphology from their normal counterpart. The HOK-16A and HOK-16B lines contained ˜40 and ˜25 copies of intact HPV-16 DNA as integrated form per cell respectively, and both cell lines exprpssed several viral specific poly(A+) RNAs. Notably these cell lines also overexpressed cellular myc proto-oncogene in comparison with the normal counterpart. However, the immortalized cell lines were not able to produce tumors in nude mice, indicating that the cells are partially transformed. The HOK-16A and HOK-16B lines are, therefore, useful for investigating the multistep molecular events of oral carcinogenesis.
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/12.9.1627