Expression Cloning and Characterization of ACC Synthase and ACC Oxidase Genes in Paeonia lactiflora

Ethylene plays an essential role in the regulation of flower senescence and abscission. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) are key enzymes in the ethylene production. The 3'-end fragment of Paeonia lactiflora ACS gene (PlACS) the full-length cDNA and ge...

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Bibliographic Details
Published in:International journal of agriculture and biology 2014-08, Vol.16 (4)
Main Authors: Zhao, Daqiu, Tao, Jun, Zhou, Chunhua, Wang, Sai
Format: Article
Language:English
Subjects:
Paeonia
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Summary:Ethylene plays an essential role in the regulation of flower senescence and abscission. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) are key enzymes in the ethylene production. The 3'-end fragment of Paeonia lactiflora ACS gene (PlACS) the full-length cDNA and genomic sequence of P. lactiflora ACO gene (PlACO) were isolated from Paeonia lactiflora for the first time. Sequence analysis indicated that PlACS was 1126 bp in length which contained a part open reading frame (ORF) of 998 bp encoding 338 amino acid residues. The full-length of PlACO cDNA was 1097 bp containing one whole ORF of 937 bp and encoding 312 amino acid residues. The genomic sequence was 1460 bp containing four exons and three introns. Sequence alignment revealed that PlACS and PlACO shared high nucleotides similarities with previously reported ACS and ACO genes in other plant species. Temporal and spatial expression analysis revealed that the expression level of PlACS increased during petals development and the expression levels in young tissues were higher than that in other tissues. Nevertheless the expression pattern of PlACO differed with PlACS and its highest expression level was in leaves tissue. PlACS might be induced by the developmental signal and PlACO could be regulated by other environmental signals. Copyright 2014 Friends Science Publishers.
ISSN:1560-8530
1814-9596