Pressurized liquid extraction in determination of polychlorinated biphenyls and organochlorine pesticides in fish samples

Pressurized liquid extraction (PLE) is a relatively new technique applicable for the extraction of persistent organic pollutants from various matrices. The main advantages of this method are short time and low consumption of extraction solvent. The effects of various operational parameters (i.e. tem...

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Bibliographic Details
Published in:Analytica chimica acta 2004-08, Vol.520 (1), p.193-200
Main Authors: Suchan, Petr, Pulkrabová, Jana, Hajšlová, Jana, Kocourek, Vladimı́r
Format: Article
Language:English
Subjects:
Accelerated solvent extraction
Analytical chemistry
Applied sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Environmental samples
Exact sciences and technology
Gas chromatographic methods
Global environmental pollution
Organochlorine pesticides
Other chromatographic methods
Pollution
Polychlorinated biphenyls
Soxhlet extraction
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Summary:Pressurized liquid extraction (PLE) is a relatively new technique applicable for the extraction of persistent organic pollutants from various matrices. The main advantages of this method are short time and low consumption of extraction solvent. The effects of various operational parameters (i.e. temperature of extraction, number of static cycles and extraction solvent mixtures) on the PLE efficiency were investigated in this study. Fish muscle tissue containing 3.2% (w/w) lipids and native polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and other related compounds was used for testing. Purification of crude extracts was carried out by gel permeation chromatography employing Bio-Beads S-X3. Identification and quantitation of target indicator PCBs and OCPs was performed by high-resolution gas chromatography (HRGC) with two parallel electron-capture detectors (ECDs). Results obtained by the optimized PLE procedure were compared with conventional Soxhlet extraction (the same extraction solvent mixtures hexane–dichloromethane (1:1 v/v) and hexane–acetone (4:1 v/v) were used). The recoveries obtained by PLE operated at 90–120 °C were either comparable to “classic” Soxhlet extraction (for higher-chlorinated PCB congeners and DDT group) or even better (for lower chlorinated analytes). The highest recoveries were obtained for three static 5 min extraction cycles.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2004.02.061