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Corpora lutea of pregnant and pseudopregnant domestic cats reveal similar steroidogenic capacities during the luteal life span
•We quantify luteal mRNA/protein expression of conventional steroidogenic enzymes.•We measure intraluteal concentration of progestogens, oestrogens and androgens.•Expression of STAR, HSD3B1, CYP19A1, HSD17B7 declines during luteal life span.•Intraluteal steroid hormone content decreases during the l...
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| Published in: | The Journal of steroid biochemistry and molecular biology 2014-10, Vol.144, p.373-381 |
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| container_title | The Journal of steroid biochemistry and molecular biology |
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| creator | Zschockelt, Lina Amelkina, Olga Siemieniuch, Marta J. Koster, Stefanie Jewgenow, Katarina Braun, Beate C. |
| description | •We quantify luteal mRNA/protein expression of conventional steroidogenic enzymes.•We measure intraluteal concentration of progestogens, oestrogens and androgens.•Expression of STAR, HSD3B1, CYP19A1, HSD17B7 declines during luteal life span.•Intraluteal steroid hormone content decreases during the luteal phase.•Luteal steroidogenic capacity is independent of reproductive cycle in domestic cats.
In domestic cats, luteal phases of pregnancy and pseudopregnancy (non-pregnant luteal phase) differ in the course and level of plasma progesterone (P4). Therefore, we assumed differences in luteal steroidogenic capacities. Here we present a comprehensive analysis of intraluteal steroid biogenesis in the domestic cat. We quantitatively measured relative mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 oxidases (CYP), hydroxysteroid dehydrogenases (HSD), steroid reductase (SRD) and enzymes involved in sulfoconjugation of steroids, i.e. sulfotransferase (SULT) and sulfatase (STS). Protein expression was analysed by Western Blot for HSD3B. Additionally, intraluteal steroid contents were determined. During the pseudopregnant luteal phase, expression of STAR (p=0.005), HSD3B1 (p |
| doi_str_mv | 10.1016/j.jsbmb.2014.08.010 |
| format | article |
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In domestic cats, luteal phases of pregnancy and pseudopregnancy (non-pregnant luteal phase) differ in the course and level of plasma progesterone (P4). Therefore, we assumed differences in luteal steroidogenic capacities. Here we present a comprehensive analysis of intraluteal steroid biogenesis in the domestic cat. We quantitatively measured relative mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 oxidases (CYP), hydroxysteroid dehydrogenases (HSD), steroid reductase (SRD) and enzymes involved in sulfoconjugation of steroids, i.e. sulfotransferase (SULT) and sulfatase (STS). Protein expression was analysed by Western Blot for HSD3B. Additionally, intraluteal steroid contents were determined. During the pseudopregnant luteal phase, expression of STAR (p=0.005), HSD3B1 (p<0.0001), CYP19A1 (p<0.0001) and HSD17B7 (p=0.008) decreased from formation of the corpus luteum (CL) onwards. HSD3B protein expression was highest in the development/maintenance stage of CL and declined during the subsequent luteal phase of pregnancy and pseudopregnancy. This was in accordance with decreasing intraluteal levels of P4, oestrogens and androgens. In contrast, expression of SRD5A1 (p<0.001) increased with progression through stages of the pseudopregnant CL, being indicative of P4 metabolism via an alternate pathway to dihydrotestosterone (DHT). Compared to the formation stage, expression of SULT1E1 was higher in all other luteal stages of pseudopregnancy (p=0.004), implying a potential sulfoconjugation of oestrogens. Expression of CYP11A1 and CYP17A1 was unaffected by the luteal stage (p>0.05), suggesting a permanent capacity of cat CL to convert progestogens via androgen and oestrogen pathways. In general, mRNA expression profiles of steroidogenic enzymes during the pregnant luteal phase reflected the pseudopregnancy profiles. Intraluteal oestrogen (p<0.0001) and androgen (p=0.008) levels were higher in the formation stage compared to the following luteal stages of pseudopregnancy. Concentrations of P4 were higher in the development/maintenance compared to the regression stages (p=0.01). We conclude that cat CL of the same histomorphological stage are characterised by identical steroidogenic capacities independently of an on-going pregnancy.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/j.jsbmb.2014.08.010</identifier><identifier>PMID: 25138635</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>3-Oxo-5-alpha-Steroid 4-Dehydrogenase - genetics ; Animals ; Aromatase - genetics ; Base Sequence ; Cats - metabolism ; Cell Cycle Proteins - genetics ; Corpus luteum ; Corpus Luteum - metabolism ; Felis catus ; Female ; Membrane Proteins - genetics ; Molecular Sequence Data ; Multienzyme Complexes - genetics ; Multienzyme Complexes - metabolism ; Phosphoproteins - genetics ; Pregnancy ; Pregnancy - metabolism ; Progesterone Reductase - genetics ; Progesterone Reductase - metabolism ; Pseudopregnancy ; S100 Proteins - genetics ; Sequence Analysis, DNA ; Steroid hormone ; Steroid Isomerases - genetics ; Steroid Isomerases - metabolism ; Steroidogenic enzyme ; Sulfotransferases - genetics</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 2014-10, Vol.144, p.373-381</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-dcd759348fa55c4a5e75dc4567ef90cd98f2ebac0d756d75536454652ccb88693</citedby><cites>FETCH-LOGICAL-c359t-dcd759348fa55c4a5e75dc4567ef90cd98f2ebac0d756d75536454652ccb88693</cites><orcidid>0000-0002-2832-1493 ; 0000-0002-5117-9659</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25138635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zschockelt, Lina</creatorcontrib><creatorcontrib>Amelkina, Olga</creatorcontrib><creatorcontrib>Siemieniuch, Marta J.</creatorcontrib><creatorcontrib>Koster, Stefanie</creatorcontrib><creatorcontrib>Jewgenow, Katarina</creatorcontrib><creatorcontrib>Braun, Beate C.</creatorcontrib><title>Corpora lutea of pregnant and pseudopregnant domestic cats reveal similar steroidogenic capacities during the luteal life span</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>•We quantify luteal mRNA/protein expression of conventional steroidogenic enzymes.•We measure intraluteal concentration of progestogens, oestrogens and androgens.•Expression of STAR, HSD3B1, CYP19A1, HSD17B7 declines during luteal life span.•Intraluteal steroid hormone content decreases during the luteal phase.•Luteal steroidogenic capacity is independent of reproductive cycle in domestic cats.
In domestic cats, luteal phases of pregnancy and pseudopregnancy (non-pregnant luteal phase) differ in the course and level of plasma progesterone (P4). Therefore, we assumed differences in luteal steroidogenic capacities. Here we present a comprehensive analysis of intraluteal steroid biogenesis in the domestic cat. We quantitatively measured relative mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 oxidases (CYP), hydroxysteroid dehydrogenases (HSD), steroid reductase (SRD) and enzymes involved in sulfoconjugation of steroids, i.e. sulfotransferase (SULT) and sulfatase (STS). Protein expression was analysed by Western Blot for HSD3B. Additionally, intraluteal steroid contents were determined. During the pseudopregnant luteal phase, expression of STAR (p=0.005), HSD3B1 (p<0.0001), CYP19A1 (p<0.0001) and HSD17B7 (p=0.008) decreased from formation of the corpus luteum (CL) onwards. HSD3B protein expression was highest in the development/maintenance stage of CL and declined during the subsequent luteal phase of pregnancy and pseudopregnancy. This was in accordance with decreasing intraluteal levels of P4, oestrogens and androgens. In contrast, expression of SRD5A1 (p<0.001) increased with progression through stages of the pseudopregnant CL, being indicative of P4 metabolism via an alternate pathway to dihydrotestosterone (DHT). Compared to the formation stage, expression of SULT1E1 was higher in all other luteal stages of pseudopregnancy (p=0.004), implying a potential sulfoconjugation of oestrogens. Expression of CYP11A1 and CYP17A1 was unaffected by the luteal stage (p>0.05), suggesting a permanent capacity of cat CL to convert progestogens via androgen and oestrogen pathways. In general, mRNA expression profiles of steroidogenic enzymes during the pregnant luteal phase reflected the pseudopregnancy profiles. Intraluteal oestrogen (p<0.0001) and androgen (p=0.008) levels were higher in the formation stage compared to the following luteal stages of pseudopregnancy. Concentrations of P4 were higher in the development/maintenance compared to the regression stages (p=0.01). We conclude that cat CL of the same histomorphological stage are characterised by identical steroidogenic capacities independently of an on-going pregnancy.</description><subject>3-Oxo-5-alpha-Steroid 4-Dehydrogenase - genetics</subject><subject>Animals</subject><subject>Aromatase - genetics</subject><subject>Base Sequence</subject><subject>Cats - metabolism</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Corpus luteum</subject><subject>Corpus Luteum - metabolism</subject><subject>Felis catus</subject><subject>Female</subject><subject>Membrane Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - genetics</subject><subject>Multienzyme Complexes - metabolism</subject><subject>Phosphoproteins - genetics</subject><subject>Pregnancy</subject><subject>Pregnancy - metabolism</subject><subject>Progesterone Reductase - genetics</subject><subject>Progesterone Reductase - metabolism</subject><subject>Pseudopregnancy</subject><subject>S100 Proteins - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Steroid hormone</subject><subject>Steroid Isomerases - genetics</subject><subject>Steroid Isomerases - metabolism</subject><subject>Steroidogenic enzyme</subject><subject>Sulfotransferases - genetics</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kMFq3DAQhkVJaTZpn6BQdOzF7siyZPnQQ1mSphDIpT0LWRpvtdiWK8mBXvLsVbJpjjkMA8M38zMfIR8Z1AyY_HKsj2mYh7oB1tagamDwhuyY6vqKNQ2ckR30EiroJJyTi5SOAMA5696R80YwriQXO_KwD3EN0dBpy2hoGOka8bCYJVOzOLom3Fx4GbkwY8reUmtyohHv0Uw0-dlPJtKUMQbvwgGXJ2I11mePibot-uVA8288pUx08iPStJrlPXk7minhh-d-SX5dX_3c31S3d99_7L_dVpaLPlfOuk70vFWjEcK2RmAnnG2F7HDswbpejQ0OxkLBZCnBZStaKRprB6Vkzy_J59PdNYY_W_lBzz5ZnCazYNiSZrIoU10LqqD8hNoYUoo46jX62cS_moF-FK-P-km8fhSvQekivmx9eg7Yhhndy85_0wX4egKwvHnvMepkPS4WnY9os3bBvxrwD3KNmAg</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Zschockelt, Lina</creator><creator>Amelkina, Olga</creator><creator>Siemieniuch, Marta J.</creator><creator>Koster, Stefanie</creator><creator>Jewgenow, Katarina</creator><creator>Braun, Beate C.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2832-1493</orcidid><orcidid>https://orcid.org/0000-0002-5117-9659</orcidid></search><sort><creationdate>20141001</creationdate><title>Corpora lutea of pregnant and pseudopregnant domestic cats reveal similar steroidogenic capacities during the luteal life span</title><author>Zschockelt, Lina ; 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In domestic cats, luteal phases of pregnancy and pseudopregnancy (non-pregnant luteal phase) differ in the course and level of plasma progesterone (P4). Therefore, we assumed differences in luteal steroidogenic capacities. Here we present a comprehensive analysis of intraluteal steroid biogenesis in the domestic cat. We quantitatively measured relative mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 oxidases (CYP), hydroxysteroid dehydrogenases (HSD), steroid reductase (SRD) and enzymes involved in sulfoconjugation of steroids, i.e. sulfotransferase (SULT) and sulfatase (STS). Protein expression was analysed by Western Blot for HSD3B. Additionally, intraluteal steroid contents were determined. During the pseudopregnant luteal phase, expression of STAR (p=0.005), HSD3B1 (p<0.0001), CYP19A1 (p<0.0001) and HSD17B7 (p=0.008) decreased from formation of the corpus luteum (CL) onwards. HSD3B protein expression was highest in the development/maintenance stage of CL and declined during the subsequent luteal phase of pregnancy and pseudopregnancy. This was in accordance with decreasing intraluteal levels of P4, oestrogens and androgens. In contrast, expression of SRD5A1 (p<0.001) increased with progression through stages of the pseudopregnant CL, being indicative of P4 metabolism via an alternate pathway to dihydrotestosterone (DHT). Compared to the formation stage, expression of SULT1E1 was higher in all other luteal stages of pseudopregnancy (p=0.004), implying a potential sulfoconjugation of oestrogens. Expression of CYP11A1 and CYP17A1 was unaffected by the luteal stage (p>0.05), suggesting a permanent capacity of cat CL to convert progestogens via androgen and oestrogen pathways. In general, mRNA expression profiles of steroidogenic enzymes during the pregnant luteal phase reflected the pseudopregnancy profiles. Intraluteal oestrogen (p<0.0001) and androgen (p=0.008) levels were higher in the formation stage compared to the following luteal stages of pseudopregnancy. Concentrations of P4 were higher in the development/maintenance compared to the regression stages (p=0.01). We conclude that cat CL of the same histomorphological stage are characterised by identical steroidogenic capacities independently of an on-going pregnancy.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25138635</pmid><doi>10.1016/j.jsbmb.2014.08.010</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2832-1493</orcidid><orcidid>https://orcid.org/0000-0002-5117-9659</orcidid></addata></record> |
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| subjects | 3-Oxo-5-alpha-Steroid 4-Dehydrogenase - genetics Animals Aromatase - genetics Base Sequence Cats - metabolism Cell Cycle Proteins - genetics Corpus luteum Corpus Luteum - metabolism Felis catus Female Membrane Proteins - genetics Molecular Sequence Data Multienzyme Complexes - genetics Multienzyme Complexes - metabolism Phosphoproteins - genetics Pregnancy Pregnancy - metabolism Progesterone Reductase - genetics Progesterone Reductase - metabolism Pseudopregnancy S100 Proteins - genetics Sequence Analysis, DNA Steroid hormone Steroid Isomerases - genetics Steroid Isomerases - metabolism Steroidogenic enzyme Sulfotransferases - genetics |
| title | Corpora lutea of pregnant and pseudopregnant domestic cats reveal similar steroidogenic capacities during the luteal life span |
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