Corpora lutea of pregnant and pseudopregnant domestic cats reveal similar steroidogenic capacities during the luteal life span

•We quantify luteal mRNA/protein expression of conventional steroidogenic enzymes.•We measure intraluteal concentration of progestogens, oestrogens and androgens.•Expression of STAR, HSD3B1, CYP19A1, HSD17B7 declines during luteal life span.•Intraluteal steroid hormone content decreases during the l...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of steroid biochemistry and molecular biology 2014-10, Vol.144, p.373-381
Main Authors: Zschockelt, Lina, Amelkina, Olga, Siemieniuch, Marta J., Koster, Stefanie, Jewgenow, Katarina, Braun, Beate C.
Format: Article
Language:English
Subjects:
3-Oxo-5-alpha-Steroid 4-Dehydrogenase - genetics
Animals
Aromatase - genetics
Base Sequence
Cats - metabolism
Cell Cycle Proteins - genetics
Corpus luteum
Corpus Luteum - metabolism
Felis catus
Female
Membrane Proteins - genetics
Molecular Sequence Data
Multienzyme Complexes - genetics
Multienzyme Complexes - metabolism
Phosphoproteins - genetics
Pregnancy
Pregnancy - metabolism
Progesterone Reductase - genetics
Progesterone Reductase - metabolism
Pseudopregnancy
S100 Proteins - genetics
Sequence Analysis, DNA
Steroid hormone
Steroid Isomerases - genetics
Steroid Isomerases - metabolism
Steroidogenic enzyme
Sulfotransferases - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•We quantify luteal mRNA/protein expression of conventional steroidogenic enzymes.•We measure intraluteal concentration of progestogens, oestrogens and androgens.•Expression of STAR, HSD3B1, CYP19A1, HSD17B7 declines during luteal life span.•Intraluteal steroid hormone content decreases during the luteal phase.•Luteal steroidogenic capacity is independent of reproductive cycle in domestic cats. In domestic cats, luteal phases of pregnancy and pseudopregnancy (non-pregnant luteal phase) differ in the course and level of plasma progesterone (P4). Therefore, we assumed differences in luteal steroidogenic capacities. Here we present a comprehensive analysis of intraluteal steroid biogenesis in the domestic cat. We quantitatively measured relative mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 oxidases (CYP), hydroxysteroid dehydrogenases (HSD), steroid reductase (SRD) and enzymes involved in sulfoconjugation of steroids, i.e. sulfotransferase (SULT) and sulfatase (STS). Protein expression was analysed by Western Blot for HSD3B. Additionally, intraluteal steroid contents were determined. During the pseudopregnant luteal phase, expression of STAR (p=0.005), HSD3B1 (p
ISSN:0960-0760
1879-1220
DOI:10.1016/j.jsbmb.2014.08.010