Progressive obesity alters ovarian folliculogenesis with impacts on pro-inflammatory and steroidogenic signaling in female mice

Diet-induced obesity induces immune cell infiltration and inflammation in peri-ovarian adipose tissue and mRNA expression of inflammatory markers in ovarian tissue. Whether these changes are associated with obesity-related ovarian dysfunction remains unknown. In the present study, qRT-PCR and Wester...

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Bibliographic Details
Published in:Biology of reproduction 2014-10, Vol.91 (4), p.86-86
Main Authors: Nteeba, Jackson, Ganesan, Shanthi, Keating, Aileen F
Format: Article
Language:English
Subjects:
Animals
Blood Glucose - metabolism
Body Weight
Cytokines - genetics
Estrous Cycle - physiology
Female
Gene Expression Regulation - physiology
Mice
NF-kappa B - genetics
NF-kappa B - metabolism
Obesity - metabolism
Ovarian Follicle - growth & development
Ovarian Follicle - physiology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal Transduction - physiology
Steroids - metabolism
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Summary:Diet-induced obesity induces immune cell infiltration and inflammation in peri-ovarian adipose tissue and mRNA expression of inflammatory markers in ovarian tissue. Whether these changes are associated with obesity-related ovarian dysfunction remains unknown. In the present study, qRT-PCR and Western blotting techniques were used to compare mRNA and protein abundance of ovarian immune cell and inflammation markers, along with NF-kappaB and steroidogenic pathway members in normal wild-type non-agouti (a/a; lean) and lethal yellow mice (KK.CG-A(y/)J; obese) at 6, 12, 18, or 24 wk of age. Our data revealed that, beginning at 12 wk of age, NF-kappaB inflammatory signaling members were elevated (P < 0.05) in obese females. Interestingly obesity had opposing and temporal effects on the steroidogenic enzyme pathway. Obesity decreased (P < 0.05) STAR protein at 12, 18, and 24 wk of age. CYP11A1 and CYP19A1 proteins were increased (P < 0.05) at 12 wk but were decreased (P < 0.05) at 18 and 24 wk. Interestingly, CYP19A1 was increased in lethal yellow mouse ovaries at 6 wk of age, potentially indicating early puberty onset. These data demonstrate that obesity alters expression of ovarian inflammatory and steroidogenic pathway genes in ways which could adversely affect ovarian function.
ISSN:1529-7268
DOI:10.1095/biolreprod.114.121343