Nitrogen-Gas Bubbling during the Cultivation of Clostridium tetani Produces a Higher Yield of Tetanus Toxin for the Preparation of Its Toxoid

We investigated the effect of exposing cultures of Clostridium tetani to nitrogen (N2) gas on the recovery of tetanus toxin to be processed for the preparation of its toxoid. N2 was bubbled through nine 10-liter cultures during the growth of the bacteria, while nine parallel control incubations were...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1997, Vol.41(2), pp.161-163
Main Authors: De Luca, María Marta, Abeiro, Héctor Dario, Bernagozzi, Jorge Alberto, Basualdo, Juan Angel
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Bioreactors
Biotechnology
Clostridium tetani
Clostridium tetani - growth & development
Clostridium tetani - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Microbiology
N2 gas
Nitrogen - metabolism
Production of active biomolecules
Tetanus
Tetanus Toxin - biosynthesis
Tetanus Toxin - isolation & purification
Tetanus Toxoid - biosynthesis
Toxoid
Vaccins
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Summary:We investigated the effect of exposing cultures of Clostridium tetani to nitrogen (N2) gas on the recovery of tetanus toxin to be processed for the preparation of its toxoid. N2 was bubbled through nine 10-liter cultures during the growth of the bacteria, while nine parallel control incubations were maintained without bubbling. We found that treatment of the C. tetani anaerobes with an inert gas in this manner during cultivation produced a highly significant increase in the yield of tetanus toxin from them in comparison with the standard procedure.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1997.tb01181.x