Stabilization of D-amino acid oxidase from yeast trigonopsis variabilis used for production of glutaryl-7-aminocephalosporanic acid from cephalosporin C

The studies to improve the production of glutaryl-7-ACA from cephalosporin C are described in this paper. During the conversion of cephalosporin C to keto-adipyl-7-aminocephalosporanic acid by D-amino acid oxidase (D-AAO), with the simultaneous production of equimolar amount of hydrogen peroxide, an...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1991-03, Vol.27 (3), p.239-250
Main Authors: SZWAJCER DEY, E, FLYGARE, S, MOSBACH, K
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cephalosporin C
D-amino-acid oxidase
Fundamental and applied biological sciences. Psychology
glutaryl-7-aminocephalospornic acid
Immobilization of enzymes and other molecules
Immobilization techniques
immobilized enzymes
Methods. Procedures. Technologies
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Summary:The studies to improve the production of glutaryl-7-ACA from cephalosporin C are described in this paper. During the conversion of cephalosporin C to keto-adipyl-7-aminocephalosporanic acid by D-amino acid oxidase (D-AAO), with the simultaneous production of equimolar amount of hydrogen peroxide, an incomplete nonenzymatic conversion of the keto form into the glutaryl form occurs, where cephalosporin C as well as D-AAO are partly destroyed in the presence of hydrogen peroxide. D-AAO was immobilized to different carriers in order to achieve better enzyme stability. The activity of immobilized D-AAO on manganese oxide remained above 100% during the first 9 h of a semicontinuous conversion of cephalosporin C.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02921538