Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast

A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M NH4OH. This resulted in the removing about 80-90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was...

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Bibliographic Details
Published in:Biotechnology letters 1996-09, Vol.18 (9), p.1013-1018
Main Authors: Cao, N.J, Krishnan, M.S, Du, J.X, Gong, C.S, Ho, N.W.Y, Chen, Z.D, Tsao, G.T
Format: Article
Language:English
Subjects:
ammonia
Biological Sciences
Saccharomyces
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Summary:A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M NH4OH. This resulted in the removing about 80-90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was obtained after enzymatic saccharification of cellulose fraction. By using a genetically engineered, xylose-fermenting Saccharomyces 1400(pLNH33) in the batch fermentation of a glucose-xylose mixture from corn cob, an ethanol concentration of 47 g/L was obtained within 36 h with 84% yield. In addition, an ethanol concentration of 45 g/L was obtained within 48 h with 86% yield using simultaneous saccharification-fermentation process.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00129723