Optimisation of a host/vector system for heterologous gene expression by Hansenula polymorpha

For the methylotrophic yeast, Hansenula polymorpha , expression vectors with different origins of replication have been constructed in order to analyse their influence on transformation and integration efficiency. The constructed plasmids are identical except for their origin of replication, which i...

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Bibliographic Details
Published in:Current genetics 1991-01, Vol.19 (2), p.81-87
Main Authors: Sierkstra, L.N. (Unilever Research Lab. Vlaardingen (Netherlands)), Verbakel, J.M.A, Verrips, C.T
Format: Article
Language:English
Subjects:
ALFA GALACTOSIDASA
ALPHA GALACTOSIDASE
Biological and medical sciences
Biotechnology
cloning vectors
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genetic engineering
Genetic technics
HANSENULA
HOST PARASITE RELATIONS
INTEGRACION
INTEGRATION
Methods. Procedures. Technologies
Methylotrophic yeast
Origin of replication
Plant alpha-galactosidase
RELACIONES HUESPED PARASITO
RELATION HOTE PARASITE
transformation
VECTEUR DE MALADIE
VECTORES
VECTORS
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:For the methylotrophic yeast, Hansenula polymorpha , expression vectors with different origins of replication have been constructed in order to analyse their influence on transformation and integration efficiency. The constructed plasmids are identical except for their origin of replication, which involve, respectively, that of the Saccharomyces cerevisiae 2- mu m plasmid and a H. polymorpha ARS sequence (HARS2). A plasmid with no origin of replication served as a control.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00326287