Effects of digoxin and Na, K-ATPase immunoexpression on human oral squamous carcinomas

The present study evaluated the expression of α1 and β1 Na,K-ATPase, as well as the effects of digoxin (DGX) on oral squamous cell carcinomas (OSCCs). Immunohistochemical expression of α1 and β1 Na,K-ATPase were evaluated in 60 patients who underwent treatment at the São João de Deus Hospital. SCC-2...

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Bibliographic Details
Published in:	Anticancer research 2014-10, Vol.34 (10), p.5397-5403
Main Authors:	Chaves, Aline Lauda Freitas, De Lima, Priscila Oliveira, Soares, João Marcos Arantes, Dos Santos, Hélio Batista, Silva, Ana Gabriela, Muniz, Luciana Vieira, Dos Santos, Fábio Vieira, Pereira, Michele Conceição, De Azambuja Ribeiro, Rosy Iara Maciel, Barbosa, Leandro Augusto
Format:	Article
Language:	eng
Subjects:	Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - mortality Carcinoma, Squamous Cell - pathology Cell Line, Tumor Cell Survival - drug effects Cell Survival - genetics Digoxin - pharmacology Enzyme Inhibitors - pharmacology Humans Mouth Neoplasms - genetics Mouth Neoplasms - metabolism Mouth Neoplasms - mortality Mouth Neoplasms - pathology Neoplasm Grading Neoplasm Staging Protein Subunits - genetics Protein Subunits - metabolism Risk Factors Sodium-Potassium-Exchanging ATPase - genetics Sodium-Potassium-Exchanging ATPase - metabolism
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Summary:	The present study evaluated the expression of α1 and β1 Na,K-ATPase, as well as the effects of digoxin (DGX) on oral squamous cell carcinomas (OSCCs). Immunohistochemical expression of α1 and β1 Na,K-ATPase were evaluated in 60 patients who underwent treatment at the São João de Deus Hospital. SCC-25 viability was assessed by the colorimetric assay. Chi-square or Fisher's exact tests were used to analyze the association of α1 and β1 Na,K-ATPase expression with the variables. Immunoexpression of α1 and β1 Na,K-ATPase were observed in 28% and 55% of the tumors, however these proteins were not significant prognostic factors. Tobacco was significantly associated with α1 expression. SCC-25 viability decreased significantly after treatment with 1 μM DGX at 24 h. The smoking status of OSCC patients was significantly associated with α1 expression and DGX affected the SCC-25 viability in a dose- and duration-dependent manner.
ISSN:	1791-7530