Somatic hybrid plants between the forage legumes Medicago sativa L. and Medicago arborea L

Interspecific somatic hybrid plants were obtained by symmetrical electrofusion of mesophyll protoplasts of Medicago sativa with callus protoplasts of Medicago arborea. Somatic hybrid calli were picked manually from semi-solid culture medium after they were identified by their dual color in fluoresce...

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Bibliographic Details
Published in:Theoretical and applied genetics 1996-07, Vol.93 (1-2), p.183-189
Main Authors: Nenz, E, Pupilli, F, Damiani, F, Arcioni, S. (Institut di Ricerche sul Miglioramento Genetico delle Piante Foraggere del C.N.R., Perugia (Italy))
Format: Article
Language:English
Subjects:
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
FUSION DE PROTOPLASTES
FUSION DEL PROTOPLASTO
Genetics of eukaryotes. Biological and molecular evolution
HIBRIDACION INTERESPECIFICA
HIBRIDOS SOMATICOS
HYBRIDATION INTERSPECIFIQUE
HYBRIDE SOMATIQUE
INTERSPECIFIC HYBRIDIZATION
MEDICAGO
MEDICAGO SATIVA
PROTOPLAST FUSION
Pteridophyta, spermatophyta
SOMATIC HYBRIDS
Vegetals
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Summary:Interspecific somatic hybrid plants were obtained by symmetrical electrofusion of mesophyll protoplasts of Medicago sativa with callus protoplasts of Medicago arborea. Somatic hybrid calli were picked manually from semi-solid culture medium after they were identified by their dual color in fluorescent light. Twelve putative hybrid calli were selected and one of them regenerated plants. The morphogenesis of the somatic hybrid calli was induced by the synthetic growth regulator 1,2 benzisoxazole-3-acetic acid. Somatic hybrid plants showed intensive genome rearrangements, as evidenced by isozyme and RFLP analysis. The morphology of somatic hybrid plants was in general intermediate between the parents. The production of hybrids by protoplast fusion between sexually incompatible Medicago species is related to the in vitro respon siveness of the parental protoplasts. The possibility of using somatic hybrid plants in alfalfa breeding is discussed.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00225744