Cell‐type specificity of anti‐CD45 autoantibodies in systemic lupus erythematosus

Objective. To determine the specificity of anti‐CD45 autoantibodies in systemic lupus erythematosus (SLE) for native CD45 and for CD45 expressed by T cells and B cells, and at different stages of cellular activation. Methods. CD45 purified from different types of lymphocytes was examined by immunobl...

Full description

Saved in:
Bibliographic Details
Published in:Arthritis and rheumatism 1996-04, Vol.39 (4), p.592-599
Main Authors: Czyzyk, Jan, Fernsten, Philip, Shaw, Melody, Winfield, John B.
Format: Article
Language:English
Subjects:
Antibody Specificity
Autoantibodies
B-Lymphocytes - immunology
Biological and medical sciences
Humans
Immunoglobulin M
Leukocyte Common Antigens - analysis
Lupus Erythematosus, Systemic - immunology
Lupus Erythematosus, Systemic - pathology
Lymphocyte Activation - immunology
Medical sciences
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
T-Lymphocytes - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective. To determine the specificity of anti‐CD45 autoantibodies in systemic lupus erythematosus (SLE) for native CD45 and for CD45 expressed by T cells and B cells, and at different stages of cellular activation. Methods. CD45 purified from different types of lymphocytes was examined by immunoblotting with sera from patients with SLE. Indirect immunofluorescence experiments were performed with purified anti‐CD45 autoantibodies. Results. IgM anti‐CD45 autoantibodies in SLE recognize native CD45 expressed on the surface membrane of viable lymphocytes and CD45 purified from activated peripheral T cells and certain T cell lines, but not CD45 purified from B cells or resting peripheral T cells. The presence or absence of reactivity is independent of the individual isoforms expressed. Conclusion. Recognition of CD45 by IgM antilymphocyte autoantibodies in SLE varies with the lineage and state of activation of the lymphocyte target. This pattern of reactivity is consistent with autoantibody specificities involving CD45 glycoforms, rather than CD45 isoforms.
ISSN:0004-3591
1529-0131
DOI:10.1002/art.1780390408