Inhibitory effect of phloretin and biochanin A on IgE-mediated allergic responses in rat basophilic leukemia RBL-2H3 cells

Anti-allergic effects and action mechanism of phloretin (Phl) and biochanin A (BioA) on the IgE–antigen complex-mediated allergic responses in rat basophilic leukemia RBL-2H3 cells were investigated. Cell viability, formation of reactive oxygen species (ROS), DPPH radical-scavenging activity, β-hexo...

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Bibliographic Details
Published in:Life sciences (1973) 2013-09, Vol.93 (9-11), p.401-408
Main Authors: Chung, Mi Ja, Sohng, Jae Kyung, Choi, Doo Jin, Park, Yong Il
Format: Article
Language:English
Subjects:
Animals
Anti-allergic activity
biochanin A
Blotting, Western
Cell Line, Tumor
cell viability
Cytokine
Cytokines - drug effects
Cytokines - metabolism
Degranulation
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Flavonoids
free radical scavengers
Free Radical Scavengers - administration & dosage
Free Radical Scavengers - pharmacology
Genistein - administration & dosage
Genistein - pharmacology
Immunoglobulin E - immunology
interleukin-13
interleukin-4
leukemia
Leukemia, Basophilic, Acute - immunology
mechanism of action
mitogen-activated protein kinase
Mitogen-Activated Protein Kinases - metabolism
Phloretin - administration & dosage
Phloretin - pharmacology
phosphorylation
Phosphorylation - drug effects
Proto-Oncogene Proteins c-akt - metabolism
Rats
RBL-2H3 cells
reactive oxygen species
Reactive Oxygen Species - metabolism
Reverse Transcriptase Polymerase Chain Reaction
tumor necrosis factor-alpha
Western blotting
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Summary:Anti-allergic effects and action mechanism of phloretin (Phl) and biochanin A (BioA) on the IgE–antigen complex-mediated allergic responses in rat basophilic leukemia RBL-2H3 cells were investigated. Cell viability, formation of reactive oxygen species (ROS), DPPH radical-scavenging activity, β-hexosaminidase release, production of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-α) and phosphorylation of Akt and mitogen-activated protein kinase (MAPK) were determined by MTT assay, 2,7-dichlorofluorescein diacetate (DCF-DA) assay, DPPH radical-scavenging assay, reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and western blot analysis, respectively. Ph1 and BioA dose-dependently inhibited the formation of ROS and the release of β-hexosaminidase from the RBL-2H3 cells and also showed DPPH radical-scavenging activity. Ph1 and BioA suppressed the antigen-induced phosphorylation of the downstream signaling intermediates, including MAPK and Akt, which are critical for the production of pro-inflammatory cytokines, and also significantly attenuated the production of IgE-mediated pro-inflammatory cytokines, such as IL-4, IL-13, and TNF-α. Phloretin and biochanin A attenuate the degranulation and allergic cytokine production through inhibition of intracellular ROS production and the phosphorylation of Akt and the MAPKs, such as ERK1/2, p38, and JNK. The results of this study suggested that these two plant flavonoids may have potent anti-allergic activity in vitro.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2013.07.019