New shrimp IgE‐binding proteins involved in mite‐seafood cross‐reactivity

SCOPE: Shrimp is a seafood consumed worldwide and the main cause of severe allergenic reactions to crustaceans. Seafood allergy has been related to mite sensitization, mainly mediated by tropomyosin, but other proteins could be involved. The aim of the study was to identify new shrimp allergens impl...

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Bibliographic Details
Published in:Molecular nutrition & food research 2014-09, Vol.58 (9), p.1915-1925
Main Authors: Gámez, Cristina, Zafra, M. Paz, Boquete, Manuel, Sanz, Verónica, Mazzeo, Carla, Ibáñez, M. Dolores, Sánchez‐García, Silvia, Sastre, Joaquín, Pozo, Victoria
Format: Article
Language:English
Subjects:
actin
Adolescent
Adult
allergenicity
Allergens
Allergens - analysis
Allergens - immunology
Allergy
Amino Acid Sequence
Animals
arginine kinase
Biological and medical sciences
Child
Child, Preschool
Climate
Cross Reactions - immunology
Cross-reactivity
Decapoda
Dermatophagoides pteronyssinus - immunology
Feeding. Feeding behavior
Female
Food Hypersensitivity - immunology
fructose-bisphosphate aldolase
Fundamental and applied biological sciences. Psychology
Galectin 3 - immunology
Humans
hypersensitivity
Immune Sera
immunoblotting
Immunoglobulin E - analysis
Male
Middle Aged
Mite seafood
mites
Mites - immunology
Molecular Sequence Data
patients
seafoods
Shellfish
Shrimp
Solenocera
Solenocera melantho
Spain
Tropomyosin - immunology
ubiquitin
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:SCOPE: Shrimp is a seafood consumed worldwide and the main cause of severe allergenic reactions to crustaceans. Seafood allergy has been related to mite sensitization, mainly mediated by tropomyosin, but other proteins could be involved. The aim of the study was to identify new shrimp allergens implicated in mite‐seafood cross‐reactivity (CR) in two different climate populations: dry and humid climates. METHODS AND RESULTS: Shrimp and mite IgE‐binding profiles of patients from continental dry and humid climates were analyzed by immunoblotting, and the most frequently recognized Solenocera melantho shrimp proteins were identified by MS as α‐actinin, β‐actin, fructose biphosphate aldolase, arginine kinase, sarcoplasmic calcium‐binding protein, and ubiquitin. Using inhibition immunoblot assays, we demonstrate that tropomyosin and ubiquitin were responsible for mite‐seafood CR from both climates; but also α‐actinin and arginine kinase are implicated in dry‐ and humid‐climate populations, respectively. Reciprocal inhibition assays demonstrated that mites are the primary sensitizer in humid‐climate, as shrimp is in continental dry‐climate population. CONCLUSION: Several new shrimp allergens have been identified and should be considered in the diagnosis and treatment of shrimp allergy and mite‐seafood CR. Differences in mite‐seafood CR were founded to be based on the climate.
ISSN:1613-4125
1613-4133
DOI:10.1002/mnfr.201400122