Simultaneous quantitation of 5- and 7-hydroxyflavone antioxidants and their binding constants with BSA using dual chiral capillary electrophoresis (dCCE) and HPLC with fluorescent detection

In this article we present two novel uses of the sensitive techniques HPLC fluorescence and dCCE for both the quantitation and binding studies of the 5- and 7-HFs extracted from the plant Alfalfa with Albumin. Ultrasonic extraction method as an extra energy source is used to enhance the extraction e...

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Bibliographic Details
Published in:Talanta (Oxford) 2014-02, Vol.119, p.417-424
Main Authors: Abo Markeb, Ahmad, Abo El-Maali, Nagwa
Format: Article
Language:English
Subjects:
Antioxidants - analysis
Binding constants
BSA
Capillary electrophoresis
Chromatography, High Pressure Liquid - methods
Electrophoresis, Capillary - methods
Flavonoids - analysis
HPLC Fluorescent detection
Monohydroxyflavones
Quantitation
Serum Albumin, Bovine - chemistry
Spectrometry, Fluorescence - methods
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Summary:In this article we present two novel uses of the sensitive techniques HPLC fluorescence and dCCE for both the quantitation and binding studies of the 5- and 7-HFs extracted from the plant Alfalfa with Albumin. Ultrasonic extraction method as an extra energy source is used to enhance the extraction efficiency and speed up. The two antioxidants could be easily separated and quantified after a 10.0-min run time. Multiple calibration curves for their analysis exhibited consistent linearity and reproducibility in the range of 0.20–2.00mgL−1 for 5-HF (r >0.9979) and 0.01–0.10mgL−1 for 7-HF (r >0.9999). Limits of Detection were 0.500µgL−1 and 0.025µgL−1 for 5-HF and 7-HF respectively. Lower Limits of Quantification were 131.600µgL−1 for 5-HF and 6.579µgL−1 for 7-HF. Inter-assay imprecision was
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2013.11.029