Autonomous bottom-up fabrication of three-dimensional nano/microcellulose honeycomb structures, directed by bacterial nanobuilder

We investigated the autonomous bottom-up fabrication of three-dimensional honeycomb cellulose structures, using Gluconacetobacter xylinus as a bacterial nanoengine, on cellulose honeycomb templates prepared by casting water-in-oil emulsions on glass substrates (Kasai and Kondo, Macromol. Biosci., 4,...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2014-10, Vol.118 (4), p.482-487
Main Authors: KONDO, Tetsuo, KASAI, Wakako
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cellulose - biosynthesis
Cellulose - chemistry
Cellulose - secretion
Emulsions
Fundamental and applied biological sciences. Psychology
Gluconacetobacter xylinus - chemistry
Gluconacetobacter xylinus - metabolism
Green Chemistry Technology
Microtechnology - methods
Nanofibers - chemistry
Nanofibers - ultrastructure
Nanotechnology - methods
Water
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Summary:We investigated the autonomous bottom-up fabrication of three-dimensional honeycomb cellulose structures, using Gluconacetobacter xylinus as a bacterial nanoengine, on cellulose honeycomb templates prepared by casting water-in-oil emulsions on glass substrates (Kasai and Kondo, Macromol. Biosci., 4, 17-21, 2004). The template film had a unique molecular orientation state along the honeycomb frames, but was non-crystalline. When G. xylinus, used as a nanofiber-producing bacterium, was incubated on the honeycomb scaffold in a culture medium, it secreted cellulose nanofibers only on the upper surface of the honeycomb frame. The movement was regulated by a selective interaction between the synthesized nanofiber and the surface of the honeycomb frames of the template. The relationship between directed deposition of synthesized nanofibers and ordered fabrication from the nano- to the micro-scale could provide a novel bottom-up methodology, using bacteria, for the design of three-dimensional honeycomb structures as functional materials with nano/micro hierarchical structures, with low energy consumption.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2014.04.002